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Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension.

Publication ,  Journal Article
Gardner, SY; McGee, JK; Kodavanti, UP; Ledbetter, A; Everitt, JI; Winsett, DW; Doerfler, DL; Costa, DL
Published in: Environ Health Perspect
June 2004

Preexistent cardiopulmonary disease in humans appears to enhance susceptibility to the adverse effects of ambient particulate matter. Previous studies in this laboratory have demonstrated enhanced inflammation and mortality after intratracheal instillation (IT) and inhalation (INH) of residual oil fly ash (ROFA) in a rat model of pulmonary hypertension induced by monocrotaline (MCT). The present study was conducted to examine the effects of ROFA in this model on ventilatory function in unanesthetized, unrestrained animals. Sixty-day-old male CD rats were injected with MCT (60 mg/kg) or vehicle (VEH) intraperitoneally 10 days before IT of ROFA (8.3 mg/kg) or saline (SAL) (control) or nose-only INH of ROFA [15 mg/m3 for 6 hr on 3 consecutive days or air (control)]. At 24 and 72 hr after exposure, rats were studied individually in a simultaneous gas uptake/whole-body plethysmograph. Lungs were removed at 72 hr for histology. Pulmonary test results showed that tidal volume (VT) decreased 24 hr after IT of ROFA in MCT-treated rats. Breathing frequency, minute volume (VE), and the ventilatory equivalent for oxygen increased in MCT- and VEH-treated rats 24 hr after IT or INH of ROFA and remained elevated 72 hr post-IT. O2 uptake (VO2) decreased after IT of ROFA in MCT-treated rats. Carbon monoxide uptake decreased 24 hr after IT of ROFA, returning to control values in VEH-treated rats but remaining low in MCT-treated rats 72 hr post-IT. ROFA exposure induced histologic changes and abnormalities in several ventilatory parameters, many of which were enhanced by MCT treatment.

Duke Scholars

Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

June 2004

Volume

112

Issue

8

Start / End Page

872 / 878

Location

United States

Related Subject Headings

  • Toxicology
  • Respiratory Function Tests
  • Rats, Sprague-Dawley
  • Rats
  • Particulate Matter
  • Particle Size
  • Monocrotaline
  • Male
  • Lung
  • Inhalation Exposure
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gardner, S. Y., McGee, J. K., Kodavanti, U. P., Ledbetter, A., Everitt, J. I., Winsett, D. W., … Costa, D. L. (2004). Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environ Health Perspect, 112(8), 872–878. https://doi.org/10.1289/ehp.6583
Gardner, Sarah Y., John K. McGee, Urmila P. Kodavanti, Allen Ledbetter, Jeffrey I. Everitt, Darrell W. Winsett, Donald L. Doerfler, and Daniel L. Costa. “Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension.Environ Health Perspect 112, no. 8 (June 2004): 872–78. https://doi.org/10.1289/ehp.6583.
Gardner SY, McGee JK, Kodavanti UP, Ledbetter A, Everitt JI, Winsett DW, et al. Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environ Health Perspect. 2004 Jun;112(8):872–8.
Gardner, Sarah Y., et al. “Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension.Environ Health Perspect, vol. 112, no. 8, June 2004, pp. 872–78. Pubmed, doi:10.1289/ehp.6583.
Gardner SY, McGee JK, Kodavanti UP, Ledbetter A, Everitt JI, Winsett DW, Doerfler DL, Costa DL. Emission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environ Health Perspect. 2004 Jun;112(8):872–878.

Published In

Environ Health Perspect

DOI

ISSN

0091-6765

Publication Date

June 2004

Volume

112

Issue

8

Start / End Page

872 / 878

Location

United States

Related Subject Headings

  • Toxicology
  • Respiratory Function Tests
  • Rats, Sprague-Dawley
  • Rats
  • Particulate Matter
  • Particle Size
  • Monocrotaline
  • Male
  • Lung
  • Inhalation Exposure