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Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis.

Publication ,  Journal Article
Cho, J; Lee, C-J; Zhao, J; Young, HE; Zhou, P
Published in: Nat Microbiol
August 15, 2016

In most Gram-negative pathogens, the hydrolysis of UDP-2,3-diacylglucosamine to generate lipid X in lipid A biosynthesis is catalysed by the membrane-associated enzyme LpxH. We report the crystal structure of LpxH in complex with its product, lipid X, unveiling a unique insertion lid above the conserved architecture of calcineurin-like phosphoesterases. This structure reveals elaborate interactions surrounding lipid X and provides molecular insights into the substrate selectivity, catalysis and inhibition of LpxH.

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Published In

Nat Microbiol

DOI

EISSN

2058-5276

Publication Date

August 15, 2016

Volume

1

Issue

11

Start / End Page

16154

Location

England

Related Subject Headings

  • Substrate Specificity
  • Pyrophosphatases
  • Models, Molecular
  • Lipid A
  • Hydrolysis
  • Haemophilus influenzae
  • Glycolipids
  • Crystallography, X-Ray
  • Crystallization
  • Carbohydrate Metabolism
 

Citation

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Cho, J., Lee, C.-J., Zhao, J., Young, H. E., & Zhou, P. (2016). Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. Nat Microbiol, 1(11), 16154. https://doi.org/10.1038/nmicrobiol.2016.154
Cho, Jae, Chul-Jin Lee, Jinshi Zhao, Hayley E. Young, and Pei Zhou. “Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis.Nat Microbiol 1, no. 11 (August 15, 2016): 16154. https://doi.org/10.1038/nmicrobiol.2016.154.
Cho J, Lee C-J, Zhao J, Young HE, Zhou P. Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. Nat Microbiol. 2016 Aug 15;1(11):16154.
Cho, Jae, et al. “Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis.Nat Microbiol, vol. 1, no. 11, Aug. 2016, p. 16154. Pubmed, doi:10.1038/nmicrobiol.2016.154.
Cho J, Lee C-J, Zhao J, Young HE, Zhou P. Structure of the essential Haemophilus influenzae UDP-diacylglucosamine pyrophosphohydrolase LpxH in lipid A biosynthesis. Nat Microbiol. 2016 Aug 15;1(11):16154.

Published In

Nat Microbiol

DOI

EISSN

2058-5276

Publication Date

August 15, 2016

Volume

1

Issue

11

Start / End Page

16154

Location

England

Related Subject Headings

  • Substrate Specificity
  • Pyrophosphatases
  • Models, Molecular
  • Lipid A
  • Hydrolysis
  • Haemophilus influenzae
  • Glycolipids
  • Crystallography, X-Ray
  • Crystallization
  • Carbohydrate Metabolism