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PC3 is a cell line characteristic of prostatic small cell carcinoma.

Publication ,  Journal Article
Tai, S; Sun, Y; Squires, JM; Zhang, H; Oh, WK; Liang, C-Z; Huang, J
Published in: Prostate
November 2011

BACKGROUND: The majority of the prostatic cancers are adenocarcinomas characterized by glandular formation and the expression of luminal differentiation markers androgen receptor (AR) and prostate-specific antigen (PSA). Most adenocarcinomas are indolent and androgen-dependent. Hormonal therapy that inhibits AR signaling produces symptomatic relief in patients with advanced and metastatic adenocarcinomas. Prostatic small cell neuroendocrine carcinoma (SCNC) is a variant form of prostate cancer (PC). In contrast to adenocarcinoma, the tumor cells of SCNC do not form glands and are negative for AR and PSA. SCNC is extremely aggressive and does not respond to hormonal therapy. The purpose of this study was to compare the important and relevant features of two most commonly used PC cell lines, LNCaP and PC3, with prostatic adenocarcinoma and SCNC. METHODS: Xenograft tumors of LNCaP and PC3 were prepared and compared with human prostatic adenocarcinoma and SCNC for the expression of key signaling molecules by immunohistochemistry and Western blot analysis. RESULTS: LNCaP cells express AR and PSA and their growth is inhibited by androgen withdrawal, similar to human prostatic adenocarcinoma. PC3 cells do not express AR and PSA and their proliferation is independent of androgen, similar to SCNC. Adenocarcinoma cells and LNCaP cells are negative for neuroendocrine markers and stem cell-associated marker CD44 while SCNC and PC3 cells are positive. LNCaP cells have identical cytokeratin profiles to adenocarcinoma while PC3 cells have cytokeratin profiles similar to SCNC. CONCLUSION: LNCaP cells share common features with adenocarcinoma while PC3 cells are characteristic of SCNC.

Duke Scholars

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Published In

Prostate

DOI

EISSN

1097-0045

Publication Date

November 2011

Volume

71

Issue

15

Start / End Page

1668 / 1679

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Androgen
  • RNA, Neoplasm
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Oncology & Carcinogenesis
  • Neoplasms, Hormone-Dependent
  • Neoplasm Transplantation
  • Mice, SCID
 

Citation

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ICMJE
MLA
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Tai, S., Sun, Y., Squires, J. M., Zhang, H., Oh, W. K., Liang, C.-Z., & Huang, J. (2011). PC3 is a cell line characteristic of prostatic small cell carcinoma. Prostate, 71(15), 1668–1679. https://doi.org/10.1002/pros.21383
Tai, Sheng, Yin Sun, Jill M. Squires, Hong Zhang, William K. Oh, Chao-Zhao Liang, and Jiaoti Huang. “PC3 is a cell line characteristic of prostatic small cell carcinoma.Prostate 71, no. 15 (November 2011): 1668–79. https://doi.org/10.1002/pros.21383.
Tai S, Sun Y, Squires JM, Zhang H, Oh WK, Liang C-Z, et al. PC3 is a cell line characteristic of prostatic small cell carcinoma. Prostate. 2011 Nov;71(15):1668–79.
Tai, Sheng, et al. “PC3 is a cell line characteristic of prostatic small cell carcinoma.Prostate, vol. 71, no. 15, Nov. 2011, pp. 1668–79. Pubmed, doi:10.1002/pros.21383.
Tai S, Sun Y, Squires JM, Zhang H, Oh WK, Liang C-Z, Huang J. PC3 is a cell line characteristic of prostatic small cell carcinoma. Prostate. 2011 Nov;71(15):1668–1679.
Journal cover image

Published In

Prostate

DOI

EISSN

1097-0045

Publication Date

November 2011

Volume

71

Issue

15

Start / End Page

1668 / 1679

Location

United States

Related Subject Headings

  • Transplantation, Heterologous
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Androgen
  • RNA, Neoplasm
  • Prostatic Neoplasms
  • Prostate-Specific Antigen
  • Oncology & Carcinogenesis
  • Neoplasms, Hormone-Dependent
  • Neoplasm Transplantation
  • Mice, SCID