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Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.

Publication ,  Journal Article
Feger, BJ; Thompson, JW; Dubois, LG; Kommaddi, RP; Foster, MW; Mishra, R; Shenoy, SK; Shibata, Y; Kidane, YH; Moseley, MA; Carnell, LS; Bowles, DE
Published in: Sci Rep
September 27, 2016

On Earth, biological systems have evolved in response to environmental stressors, interactions dictated by physical forces that include gravity. The absence of gravity is an extreme stressor and the impact of its absence on biological systems is ill-defined. Astronauts who have spent extended time under conditions of minimal gravity (microgravity) experience an array of biological alterations, including perturbations in cardiovascular function. We hypothesized that physiological perturbations in cardiac function in microgravity may be a consequence of alterations in molecular and organellar dynamics within the cellular milieu of cardiomyocytes. We used a combination of mass spectrometry-based approaches to compare the relative abundance and turnover rates of 848 and 196 proteins, respectively, in rat neonatal cardiomyocytes exposed to simulated microgravity or normal gravity. Gene functional enrichment analysis of these data suggested that the protein content and function of the mitochondria, ribosomes, and endoplasmic reticulum were differentially modulated in microgravity. We confirmed experimentally that in microgravity protein synthesis was decreased while apoptosis, cell viability, and protein degradation were largely unaffected. These data support our conclusion that in microgravity cardiomyocytes attempt to maintain mitochondrial homeostasis at the expense of protein synthesis. The overall response to this stress may culminate in cardiac muscle atrophy.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

September 27, 2016

Volume

6

Start / End Page

34091

Location

England
 

Citation

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Feger, B. J., Thompson, J. W., Dubois, L. G., Kommaddi, R. P., Foster, M. W., Mishra, R., … Bowles, D. E. (2016). Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection. Sci Rep, 6, 34091. https://doi.org/10.1038/srep34091
Feger, Bryan J., J Will Thompson, Laura G. Dubois, Reddy P. Kommaddi, Matthew W. Foster, Rajashree Mishra, Sudha K. Shenoy, et al. “Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.Sci Rep 6 (September 27, 2016): 34091. https://doi.org/10.1038/srep34091.
Feger BJ, Thompson JW, Dubois LG, Kommaddi RP, Foster MW, Mishra R, et al. Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection. Sci Rep. 2016 Sep 27;6:34091.
Feger, Bryan J., et al. “Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection.Sci Rep, vol. 6, Sept. 2016, p. 34091. Pubmed, doi:10.1038/srep34091.
Feger BJ, Thompson JW, Dubois LG, Kommaddi RP, Foster MW, Mishra R, Shenoy SK, Shibata Y, Kidane YH, Moseley MA, Carnell LS, Bowles DE. Microgravity induces proteomics changes involved in endoplasmic reticulum stress and mitochondrial protection. Sci Rep. 2016 Sep 27;6:34091.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

September 27, 2016

Volume

6

Start / End Page

34091

Location

England