Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.
Publication
, Journal Article
Hung, WY; Tourian, A
Published in: Biochem J
May 15, 1981
The hypothesis that there is impaired endogenous synthesis of glucosamine 6-phosphate in Huntington's-chorea fibroblasts was tested by double labelling matched pairs of fibroblasts in culture with carrier-free H3 32PO4 and [U-14C]glucosamine. The [32P]UDP-N-acetyl[14C]glucosamine and [14C]glucosamine 6-[32P]phosphate of the cellular soluble fraction was isolated by charcoal column and paper chromatography. There is no quantitative difference in 32P but a significant difference in 14C in these two sugars in a ratio of approx. 1.5 for Huntington's-chorea fibroblasts compared with normal fibroblasts.
Duke Scholars
Published In
Biochem J
DOI
ISSN
0264-6021
Publication Date
May 15, 1981
Volume
196
Issue
2
Start / End Page
495 / 498
Location
England
Related Subject Headings
- Uridine Diphosphate Sugars
- Uridine Diphosphate N-Acetylglucosamine
- Skin
- Phosphorus Radioisotopes
- Nucleotides
- Huntington Disease
- Humans
- Hexosamines
- Glucosamine
- Fibroblasts
Citation
APA
Chicago
ICMJE
MLA
NLM
Hung, W. Y., & Tourian, A. (1981). Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts. Biochem J, 196(2), 495–498. https://doi.org/10.1042/bj1960495
Hung, W. Y., and A. Tourian. “Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.” Biochem J 196, no. 2 (May 15, 1981): 495–98. https://doi.org/10.1042/bj1960495.
Hung WY, Tourian A. Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts. Biochem J. 1981 May 15;196(2):495–8.
Hung, W. Y., and A. Tourian. “Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts.” Biochem J, vol. 196, no. 2, May 1981, pp. 495–98. Pubmed, doi:10.1042/bj1960495.
Hung WY, Tourian A. Differential labelling of UDP-N-acetylglucosamine in Huntington's-chorea fibroblasts. Biochem J. 1981 May 15;196(2):495–498.
Published In
Biochem J
DOI
ISSN
0264-6021
Publication Date
May 15, 1981
Volume
196
Issue
2
Start / End Page
495 / 498
Location
England
Related Subject Headings
- Uridine Diphosphate Sugars
- Uridine Diphosphate N-Acetylglucosamine
- Skin
- Phosphorus Radioisotopes
- Nucleotides
- Huntington Disease
- Humans
- Hexosamines
- Glucosamine
- Fibroblasts