Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.
Publication
, Journal Article
Bilska, M; Tang, H; Montefiori, DC
Published in: AIDS Res Hum Retroviruses
April 2017
Env-pseudotyped viruses are valuable reagents for studies of HIV-1 neutralizing antibodies. It is often assumed that all pseudovirus particles are capable of only a single round of infection, making them a safe alternative to work with live HIV-1. In this study, we show that some Env-pseudotyped virus preparations give rise to low levels of replication-competent virus. These levels did not compromise results in the TZM-bl neutralization assay; however, their presence highlights a need to adhere to the same level of biosafety when working with Env-pseudotyped viruses that are required for work with replication competent HIV-1.
Duke Scholars
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Published In
AIDS Res Hum Retroviruses
DOI
EISSN
1931-8405
Publication Date
April 2017
Volume
33
Issue
4
Start / End Page
368 / 372
Location
United States
Related Subject Headings
- env Gene Products, Human Immunodeficiency Virus
- Virus Replication
- Virulence
- Virology
- Retroviridae
- Neutralization Tests
- Humans
- Containment of Biohazards
- 3202 Clinical sciences
- 1103 Clinical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Bilska, M., Tang, H., & Montefiori, D. C. (2017). Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations. AIDS Res Hum Retroviruses, 33(4), 368–372. https://doi.org/10.1089/AID.2016.0142
Bilska, Miroslawa, Haili Tang, and David C. Montefiori. “Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.” AIDS Res Hum Retroviruses 33, no. 4 (April 2017): 368–72. https://doi.org/10.1089/AID.2016.0142.
Bilska M, Tang H, Montefiori DC. Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations. AIDS Res Hum Retroviruses. 2017 Apr;33(4):368–72.
Bilska, Miroslawa, et al. “Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations.” AIDS Res Hum Retroviruses, vol. 33, no. 4, Apr. 2017, pp. 368–72. Pubmed, doi:10.1089/AID.2016.0142.
Bilska M, Tang H, Montefiori DC. Short Communication: Potential Risk of Replication-Competent Virus in HIV-1 Env-Pseudotyped Virus Preparations. AIDS Res Hum Retroviruses. 2017 Apr;33(4):368–372.
Published In
AIDS Res Hum Retroviruses
DOI
EISSN
1931-8405
Publication Date
April 2017
Volume
33
Issue
4
Start / End Page
368 / 372
Location
United States
Related Subject Headings
- env Gene Products, Human Immunodeficiency Virus
- Virus Replication
- Virulence
- Virology
- Retroviridae
- Neutralization Tests
- Humans
- Containment of Biohazards
- 3202 Clinical sciences
- 1103 Clinical Sciences