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Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells.

Publication ,  Journal Article
Gilner, JB; Walton, WG; Gush, K; Kirby, SL
Published in: Stem Cells
February 2007

Hematopoietic stem cells (HSCs) have enormous potential for use in transplantation and gene therapy. However, the frequency of repopulating HSCs is often very low; thus, highly effective techniques for cell enrichment and maintenance are required to obtain sufficient cell numbers for therapeutic use and for studies of HSC physiology. Common methods of HSC enrichment use antibodies recognizing HSC surface marker antigens. Because antibodies are known to alter the physiology of other cell types, we investigated the effect of such enrichment strategies on the physiology and lineage commitment of HSCs. We sorted HSCs using a method that does not require antibodies: exclusion of Hoechst 33342 to isolate side population (SP) cells. To elucidate the effect of antibody binding on this HSC population, we compared untreated SP cells with SP cells treated with the Sca-1(+)c-Kit(+)Lin(-) (SKL) antibody cocktail prior to SP sorting. Our findings revealed that HSCs incubated with the antibody cocktail had decreased expression of the stem cell-associated genes c-Kit, Cd34, Tal-1, and Slamf1 relative to untreated SP cells or to cells treated with polyclonal isotype control antibodies. Moreover, SKL antibodies induced cycling in SP cells and diminished their ability to confer long-term hematopoietic engraftment in lethally irradiated mice. Taken together, these data suggest that antibody-based stem cell isolation procedures can have negative effects on HSC physiology.

Duke Scholars

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

February 2007

Volume

25

Issue

2

Start / End Page

279 / 288

Location

England

Related Subject Headings

  • Whole-Body Irradiation
  • Stem Cells
  • RNA, Messenger
  • Proto-Oncogene Proteins c-kit
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Hematopoietic Stem Cell Transplantation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gilner, J. B., Walton, W. G., Gush, K., & Kirby, S. L. (2007). Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells. Stem Cells, 25(2), 279–288. https://doi.org/10.1634/stemcells.2006-0076
Gilner, Jennifer B., William G. Walton, Kimberly Gush, and Suzanne L. Kirby. “Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells.Stem Cells 25, no. 2 (February 2007): 279–88. https://doi.org/10.1634/stemcells.2006-0076.
Gilner JB, Walton WG, Gush K, Kirby SL. Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells. Stem Cells. 2007 Feb;25(2):279–88.
Gilner, Jennifer B., et al. “Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells.Stem Cells, vol. 25, no. 2, Feb. 2007, pp. 279–88. Pubmed, doi:10.1634/stemcells.2006-0076.
Gilner JB, Walton WG, Gush K, Kirby SL. Antibodies to stem cell marker antigens reduce engraftment of hematopoietic stem cells. Stem Cells. 2007 Feb;25(2):279–288.

Published In

Stem Cells

DOI

ISSN

1066-5099

Publication Date

February 2007

Volume

25

Issue

2

Start / End Page

279 / 288

Location

England

Related Subject Headings

  • Whole-Body Irradiation
  • Stem Cells
  • RNA, Messenger
  • Proto-Oncogene Proteins c-kit
  • Nuclear Proteins
  • Nerve Tissue Proteins
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Hematopoietic Stem Cell Transplantation