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Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel.

Publication ,  Journal Article
Meilander, NJ; Pasumarthy, MK; Kowalczyk, TH; Cooper, MJ; Bellamkonda, RV
Published in: Journal of controlled release : official journal of the Controlled Release Society
March 2003

Non-viral gene therapy typically results in low transfection efficiencies and transient gene expression. To address these limitations, two sustained delivery systems capable of releasing functional, compacted DNA for over 50 days were designed. A luciferase plasmid was compacted with a polylysine-polyethylene glycol conjugate and released from agarose hydrogel and lipid microtubule-hydrogel delivery systems for over 50 days. The released DNA was characterized structurally using sedimentation, electron microscopy, and serum stability, and functionally using in vitro transfections. The released DNA retained its physical compaction and nuclease resistance and was converted from supercoiled to nicked and linear forms. Released compacted DNA produced significant gene expression in vitro, although at lower levels than freshly compacted DNA. Thus, hydrogels and lipid microtubules successfully provided the slow release of bioactive, compacted DNA.

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Published In

Journal of controlled release : official journal of the Controlled Release Society

DOI

EISSN

1873-4995

ISSN

0168-3659

Publication Date

March 2003

Volume

88

Issue

2

Start / End Page

321 / 331

Related Subject Headings

  • Transfection
  • Sepharose
  • Plasmids
  • Pharmacology & Pharmacy
  • Microscopy, Electron
  • Lipids
  • In Vitro Techniques
  • Hydrogels
  • Humans
  • Gene Transfer Techniques
 

Citation

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ICMJE
MLA
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Meilander, N. J., Pasumarthy, M. K., Kowalczyk, T. H., Cooper, M. J., & Bellamkonda, R. V. (2003). Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. Journal of Controlled Release : Official Journal of the Controlled Release Society, 88(2), 321–331. https://doi.org/10.1016/s0168-3659(03)00007-5
Meilander, Nancy J., Murali K. Pasumarthy, Tomasz H. Kowalczyk, Mark J. Cooper, and Ravi V. Bellamkonda. “Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel.Journal of Controlled Release : Official Journal of the Controlled Release Society 88, no. 2 (March 2003): 321–31. https://doi.org/10.1016/s0168-3659(03)00007-5.
Meilander NJ, Pasumarthy MK, Kowalczyk TH, Cooper MJ, Bellamkonda RV. Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. Journal of controlled release : official journal of the Controlled Release Society. 2003 Mar;88(2):321–31.
Meilander, Nancy J., et al. “Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel.Journal of Controlled Release : Official Journal of the Controlled Release Society, vol. 88, no. 2, Mar. 2003, pp. 321–31. Epmc, doi:10.1016/s0168-3659(03)00007-5.
Meilander NJ, Pasumarthy MK, Kowalczyk TH, Cooper MJ, Bellamkonda RV. Sustained release of plasmid DNA using lipid microtubules and agarose hydrogel. Journal of controlled release : official journal of the Controlled Release Society. 2003 Mar;88(2):321–331.
Journal cover image

Published In

Journal of controlled release : official journal of the Controlled Release Society

DOI

EISSN

1873-4995

ISSN

0168-3659

Publication Date

March 2003

Volume

88

Issue

2

Start / End Page

321 / 331

Related Subject Headings

  • Transfection
  • Sepharose
  • Plasmids
  • Pharmacology & Pharmacy
  • Microscopy, Electron
  • Lipids
  • In Vitro Techniques
  • Hydrogels
  • Humans
  • Gene Transfer Techniques