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Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures.

Publication ,  Journal Article
Balgude, AP; Yu, X; Szymanski, A; Bellamkonda, RV
Published in: Biomaterials
May 2001

The optimization of scaffold mechanical properties for neurite extension is critical for neural tissue engineering. Agarose hydrogels can be used to stimulate and maintain three-dimensional neurite extension from primary sensory ganglia in vitro. The present study explores the structure-function relationship between dorsal root ganglion (DRG) neurite extension and agarose gel mechanical properties. A range of agarose gels of differing concentrations were generated and the corresponding rate of E9 DRG neurite extension was measured. Rate of neurite extension was inversely correlated to the mechanical stiffness of agarose gels in the range of 0.75-2.00% (wt/vol) gel concentrations. In addition, we postulate a physical model that predicts the rate of neurite extension in agarose gels, if gel stiffness is a known parameter. This model is based on Heidemann and Buxbaum's model of neurite extension. These results, if extended to scaffolds of other morphological and chemical features, would contribute significantly to the design criteria of three-dimensional scaffolds for neural tissue engineering.

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Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

May 2001

Volume

22

Issue

10

Start / End Page

1077 / 1084

Related Subject Headings

  • Sepharose
  • Neurites
  • Nerve Regeneration
  • Models, Neurological
  • Materials Testing
  • Gels
  • Ganglia, Spinal
  • Chick Embryo
  • Cells, Cultured
  • Biomedical Engineering
 

Citation

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Balgude, A. P., Yu, X., Szymanski, A., & Bellamkonda, R. V. (2001). Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials, 22(10), 1077–1084. https://doi.org/10.1016/s0142-9612(00)00350-1
Balgude, A. P., X. Yu, A. Szymanski, and R. V. Bellamkonda. “Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures.Biomaterials 22, no. 10 (May 2001): 1077–84. https://doi.org/10.1016/s0142-9612(00)00350-1.
Balgude AP, Yu X, Szymanski A, Bellamkonda RV. Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials. 2001 May;22(10):1077–84.
Balgude, A. P., et al. “Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures.Biomaterials, vol. 22, no. 10, May 2001, pp. 1077–84. Epmc, doi:10.1016/s0142-9612(00)00350-1.
Balgude AP, Yu X, Szymanski A, Bellamkonda RV. Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials. 2001 May;22(10):1077–1084.
Journal cover image

Published In

Biomaterials

DOI

EISSN

1878-5905

ISSN

0142-9612

Publication Date

May 2001

Volume

22

Issue

10

Start / End Page

1077 / 1084

Related Subject Headings

  • Sepharose
  • Neurites
  • Nerve Regeneration
  • Models, Neurological
  • Materials Testing
  • Gels
  • Ganglia, Spinal
  • Chick Embryo
  • Cells, Cultured
  • Biomedical Engineering