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The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold.

Publication ,  Journal Article
Dillon, GP; Yu, X; Sridharan, A; Ranieri, JP; Bellamkonda, RV
Published in: Journal of biomaterials science. Polymer edition
January 1998

Understanding neural cell differentiation and neurite extension in three-dimensional scaffolds is critical for neural tissue engineering. This study explores the structure-function relationship between a 3D hydrogel scaffold and neural cell process extension and examines the role of ambient charge on neurite extension in 3D scaffolds. A range of agarose hydrogel concentrations was used to generate varied gel physical structures and the corresponding neurite extension was examined. Agarose gel concentration and the corresponding pore radius are important physical properties that influence neural cell function. The average pore radii of the gels were determined while the gel was in the hydrated state and in two different dehydrated states. As the gel concentration was increased, the average pore radius decreased exponentially. Similarly, the length of neurites extended by E9 chick DRGs cultured in agarose gels depends on gel concentration. The polycationic polysaccharide chitosan and the polyanionic polysaccharide alginate were used to incorporate charge into the 3D hydrogel scaffold, and neural cell response to charge was studied. Chitosan and alginate were covalently bound to the agarose hydrogel backbone using the bi-functional coupling agent 1,1'-carbonyldiimidazole. DRGs cultured in chitosan-coupled agarose gel exhibited a significant increase in neurite length compared to the unmodified agarose control. Conversely, the alginate-coupled agarose gels significantly inhibited neurite extension. This study demonstrates a strong, correlation between the ability of sensory ganglia to extend neurites in 3D gels and the hydrogel pore radius. In addition, our results demonstrate that charged biopolymers influence neurite extension in a polarity dependent manner.

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Published In

Journal of biomaterials science. Polymer edition

DOI

EISSN

1568-5624

ISSN

0920-5063

Publication Date

January 1998

Volume

9

Issue

10

Start / End Page

1049 / 1069

Related Subject Headings

  • Sepharose
  • Neurons
  • Neurites
  • Nerve Regeneration
  • Hydrogels
  • Ganglia, Spinal
  • Dose-Response Relationship, Drug
  • Culture Techniques
  • Chitosan
  • Chitin
 

Citation

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Dillon, G. P., Yu, X., Sridharan, A., Ranieri, J. P., & Bellamkonda, R. V. (1998). The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. Journal of Biomaterials Science. Polymer Edition, 9(10), 1049–1069. https://doi.org/10.1163/156856298x00325
Dillon, G. P., X. Yu, A. Sridharan, J. P. Ranieri, and R. V. Bellamkonda. “The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold.Journal of Biomaterials Science. Polymer Edition 9, no. 10 (January 1998): 1049–69. https://doi.org/10.1163/156856298x00325.
Dillon GP, Yu X, Sridharan A, Ranieri JP, Bellamkonda RV. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. Journal of biomaterials science Polymer edition. 1998 Jan;9(10):1049–69.
Dillon, G. P., et al. “The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold.Journal of Biomaterials Science. Polymer Edition, vol. 9, no. 10, Jan. 1998, pp. 1049–69. Epmc, doi:10.1163/156856298x00325.
Dillon GP, Yu X, Sridharan A, Ranieri JP, Bellamkonda RV. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. Journal of biomaterials science Polymer edition. 1998 Jan;9(10):1049–1069.

Published In

Journal of biomaterials science. Polymer edition

DOI

EISSN

1568-5624

ISSN

0920-5063

Publication Date

January 1998

Volume

9

Issue

10

Start / End Page

1049 / 1069

Related Subject Headings

  • Sepharose
  • Neurons
  • Neurites
  • Nerve Regeneration
  • Hydrogels
  • Ganglia, Spinal
  • Dose-Response Relationship, Drug
  • Culture Techniques
  • Chitosan
  • Chitin