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Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer.

Publication ,  Journal Article
Hu, CY; Zhang, YH; Wang, T; Chen, L; Gong, ZH; Wan, YS; Li, QJ; Li, YS; Zhu, B
Published in: Clin Exp Immunol
October 2016

Malignant pleural effusion (MPE) is a poor prognostic sign for cancer patients, whereas the functional condition of MPE CD8(+) T cells is unknown. Intracavitary immunotherapy with interleukin (IL)-2 has been proven effective in controlling MPE. To elucidate the underlying mechanism, 35 lung cancer (LC) patients with MPE and 12 healthy donors were included in this study. For the IL-2 therapy experiments, after draining partial MPE, we treated 14 patients by administrating IL-2 (3 or 5 × 10(6) U in 50 ml saline) into the thoracic cavity. Before and after IL-2 treatment (40-48 h), the MPE and peripheral blood (PB) were obtained from the subjects. PB from healthy volunteers was collected as control. The expression of programmed cell death 1 (PD-1), granzyme B (GzmB), interferon (IFN)-γ and the proliferation were analysed in CD8(+) T cells from MPE and PB. The CD8(+) T cells in the MPE of LC patients showed lowest GzmB, IFN-γ and proliferation but highest PD-1 expression, compared with that in PB of LC patients and healthy donors. IL-2 treatment reduced the expression of PD-1, increased the expression of GzmB and IFN-γ and enhanced the proliferation of CD8(+) T cells in MPE. In addition, IL-2 treatment reduced carcino-embryonic antigen (CEA) level in MPE. These results indicate that MPE CD8(+) T cells exhibit exhaustion phenotype which can be reversed by IL-2 therapy.

Duke Scholars

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Published In

Clin Exp Immunol

DOI

EISSN

1365-2249

Publication Date

October 2016

Volume

186

Issue

1

Start / End Page

106 / 114

Location

England

Related Subject Headings

  • Pleural Effusion, Malignant
  • Lymphocyte Activation
  • Lung Neoplasms
  • Interleukin-2
  • Immunology
  • Humans
  • Cell Line, Tumor
  • CD8-Positive T-Lymphocytes
  • Biomarkers, Tumor
  • 3204 Immunology
 

Citation

APA
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ICMJE
MLA
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Hu, C. Y., Zhang, Y. H., Wang, T., Chen, L., Gong, Z. H., Wan, Y. S., … Zhu, B. (2016). Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer. Clin Exp Immunol, 186(1), 106–114. https://doi.org/10.1111/cei.12845
Hu, C. Y., Y. H. Zhang, T. Wang, L. Chen, Z. H. Gong, Y. S. Wan, Q. J. Li, Y. S. Li, and B. Zhu. “Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer.Clin Exp Immunol 186, no. 1 (October 2016): 106–14. https://doi.org/10.1111/cei.12845.
Hu CY, Zhang YH, Wang T, Chen L, Gong ZH, Wan YS, et al. Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer. Clin Exp Immunol. 2016 Oct;186(1):106–14.
Hu, C. Y., et al. “Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer.Clin Exp Immunol, vol. 186, no. 1, Oct. 2016, pp. 106–14. Pubmed, doi:10.1111/cei.12845.
Hu CY, Zhang YH, Wang T, Chen L, Gong ZH, Wan YS, Li QJ, Li YS, Zhu B. Interleukin-2 reverses CD8(+) T cell exhaustion in clinical malignant pleural effusion of lung cancer. Clin Exp Immunol. 2016 Oct;186(1):106–114.
Journal cover image

Published In

Clin Exp Immunol

DOI

EISSN

1365-2249

Publication Date

October 2016

Volume

186

Issue

1

Start / End Page

106 / 114

Location

England

Related Subject Headings

  • Pleural Effusion, Malignant
  • Lymphocyte Activation
  • Lung Neoplasms
  • Interleukin-2
  • Immunology
  • Humans
  • Cell Line, Tumor
  • CD8-Positive T-Lymphocytes
  • Biomarkers, Tumor
  • 3204 Immunology