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Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation.

Publication ,  Journal Article
Singleton, KD; Wischmeyer, PE
Published in: Biochem Biophys Res Commun
August 29, 2008

Glutamine (GLN) can inhibit NF-kBeta activation and cytokine expression following sepsis. NF-kappaB activation and inflammatory cytokine expression, depend on neddylation of Cullin-1 (Cul-1) to proceed. Our aim was to evaluate whether GLN inhibits Cul-1 neddylation, and further determine if GLN-mediated Cul-1 deneddylation attenuates NF-kappaB activation and subsequent cytokine expression following experimental sepsis in the mouse. Sepsis-induced via cecal ligation and puncture (CLP) led to a significant increase in lung Cul-1 neddylation. GLN administration post-sepsis led to enhanced lung Cul-1 deneddylation and attenuated NEDD8 expression (p<0.01 vs. saline). Cul-1 deneddylation was associated with decreased NF-kappaB activation and IkappaB alpha degradation in GLN treated mice (( *)p<0.01 vs. saline). Lastly, GLN treatment led to a significant decrease in lung TNF-alpha and IL-6 post-sepsis. These are the first data describing a direct effect of GLN on Cul-1 deneddylation and provide a possible mechanistic explanation for GLN's anti-inflammatory effects.

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Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

August 29, 2008

Volume

373

Issue

3

Start / End Page

445 / 449

Location

United States

Related Subject Headings

  • Sepsis
  • NF-kappa B
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lung
  • Glutamine
  • Cytokines
  • Cullin Proteins
  • Biochemistry & Molecular Biology
 

Citation

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Singleton, K. D., & Wischmeyer, P. E. (2008). Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation. Biochem Biophys Res Commun, 373(3), 445–449. https://doi.org/10.1016/j.bbrc.2008.06.057
Singleton, Kristen D., and Paul E. Wischmeyer. “Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation.Biochem Biophys Res Commun 373, no. 3 (August 29, 2008): 445–49. https://doi.org/10.1016/j.bbrc.2008.06.057.
Singleton KD, Wischmeyer PE. Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation. Biochem Biophys Res Commun. 2008 Aug 29;373(3):445–9.
Singleton, Kristen D., and Paul E. Wischmeyer. “Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation.Biochem Biophys Res Commun, vol. 373, no. 3, Aug. 2008, pp. 445–49. Pubmed, doi:10.1016/j.bbrc.2008.06.057.
Singleton KD, Wischmeyer PE. Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation. Biochem Biophys Res Commun. 2008 Aug 29;373(3):445–449.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

EISSN

1090-2104

Publication Date

August 29, 2008

Volume

373

Issue

3

Start / End Page

445 / 449

Location

United States

Related Subject Headings

  • Sepsis
  • NF-kappa B
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Lung
  • Glutamine
  • Cytokines
  • Cullin Proteins
  • Biochemistry & Molecular Biology