cAMP response element-binding protein 1 feedback loop is necessary for consolidation of long-term synaptic facilitation in Aplysia.
The transcription factor cAMP response element (CRE)-binding protein (CREB) plays an essential role in the induction of many forms of long-term synaptic plasticity. Levels of CREB1, the Aplysia homolog of CREB, show sustained elevations for several hours after the induction of long-term synaptic facilitation (LTF). Furthermore, CREB1 binds to the promoter of its own gene. These results suggest the existence of a CREB1-positive feedback loop that contributes to the consolidation of LTF. In the present study, we provide a detailed, quantitative characterization of the dynamics of CREB1 mRNA and protein as well as CREB1 phosphorylation after LTF induction. Injections of CRE oligonucleotides prevented the increase in CREB1 in response to 5-HT, corroborating the existence of the CREB1 feedback loop. This loop probably sustains CRE-dependent gene transcription, which remains elevated for at least 12 h after LTF induction. LTF is blocked by injection of CREB1 antibody after the induction phase, suggesting that the CREB1-positive feedback is required for consolidation of LTF.
Duke Scholars
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- Synapses
- Structural Homology, Protein
- Neurons, Afferent
- Neurology & Neurosurgery
- Nerve Tissue Proteins
- Long-Term Potentiation
- Feedback, Physiological
- Cyclic AMP Response Element-Binding Protein
- Coculture Techniques
- Aplysia
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Synapses
- Structural Homology, Protein
- Neurons, Afferent
- Neurology & Neurosurgery
- Nerve Tissue Proteins
- Long-Term Potentiation
- Feedback, Physiological
- Cyclic AMP Response Element-Binding Protein
- Coculture Techniques
- Aplysia