Differences in the lipid pattern of T-tube and gallbladder puncture bile in surgical patients

Summary1.The lipid composition of human bile obtained by T-tube drainage has been compared to bile obtained by direct sterile needle puncture of the gallbladder during elective surgery. The following lipids were found (mg/100 mg/dry weight) in T-tube and gallbladder bile: triglycerides (1.33 and 0.43), free fatty acids (0.76 and 0.53), free cholesterol (14.39 and 9.15), diglycerides (2.39 and 1.11), monoglycerides (1.25 and 2.34) phosphatidic acid (8.06 and 6.65), cephalin (4.70 and 5.21), lecithin (62.37 and 70.00), sphingomyelin (1.83 and 2.80), and lysolecithin 1.07 and 2.58) respectively.2.The cephalin fraction was fractionated into its two constituents, phosphatidyl ethanolamine and phosphatidyl serine and their concentration in cephalin was 25% and 75% respectively.3.Differences were demonstrated in the fatty acid composition of phosphatidyl ethanolamine and phosphatidyl serine.4.Significant differences were found in the lipid composition of bile obtained from T-tube drainage compared to direct puncture of the gallbladder in situ. T-tube specimens are readily available for study and are the major source of material chosen for any study of bile in man. On the basis of this work, caution must be exercised in interpretation of results of analyses of such material. © 1968.

Duke Scholars

Author Uma Kant Misra Pathology