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Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes.

Publication ,  Journal Article
Fujisawa, T; Wang, K; Niu, X-L; Egginton, S; Ahmad, S; Hewett, P; Kontos, CD; Ahmed, A
Published in: Cardiovasc Res
January 2017

AIMS: Atherosclerosis is a chronic inflammatory disease occurring within the artery wall. A crucial step in atherogenesis is the infiltration and retention of monocytes into the subendothelial space of large arteries induced by chemokines and growth factors. Angiopoietin-1 (Ang-1) regulates angiogenesis and reduces vascular permeability and has also been reported to promote monocyte migration in vitro. We investigated the role of Ang-1 in atherosclerosis-prone apolipoprotein-E (Apo-E) knockout mouse. METHODS AND RESULTS: Apo-E knockout (Apo-E-/-) mice fed a western or normal chow diet received a single iv injection of adenovirus encoding Ang-1 or control vector. Adenovirus-mediated systemic expression of Ang-1 induced a significant increase in early atherosclerotic lesion size and monocyte/macrophage accumulation compared with control animals receiving empty vector. Ang-1 significantly increased plasma MCP-1 and VEGF levels as measured by ELISA. FACS analysis showed that Ang-1 selectively increased inflammatory Gr1+ monocytes in the circulation, while the cell-surface expression of CD11b, which mediates monocyte emigration, was significantly reduced. CONCLUSIONS: Ang-1 specifically increases circulating Gr1+ inflammatory monocytes and increases monocyte/macrophage retention in atherosclerotic plaques, thereby contributing to development of atherosclerosis.

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Published In

Cardiovasc Res

DOI

EISSN

1755-3245

Publication Date

January 2017

Volume

113

Issue

1

Start / End Page

81 / 89

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Culture Techniques
  • Signal Transduction
  • Plaque, Atherosclerotic
  • Phenotype
  • Monocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Male
  • Humans
 

Citation

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Chicago
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Fujisawa, T., Wang, K., Niu, X.-L., Egginton, S., Ahmad, S., Hewett, P., … Ahmed, A. (2017). Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes. Cardiovasc Res, 113(1), 81–89. https://doi.org/10.1093/cvr/cvw223
Fujisawa, Takeshi, Keqing Wang, Xi-Lin Niu, Stuart Egginton, Shakil Ahmad, Peter Hewett, Christopher D. Kontos, and Asif Ahmed. “Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes.Cardiovasc Res 113, no. 1 (January 2017): 81–89. https://doi.org/10.1093/cvr/cvw223.
Fujisawa T, Wang K, Niu X-L, Egginton S, Ahmad S, Hewett P, et al. Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes. Cardiovasc Res. 2017 Jan;113(1):81–9.
Fujisawa, Takeshi, et al. “Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes.Cardiovasc Res, vol. 113, no. 1, Jan. 2017, pp. 81–89. Pubmed, doi:10.1093/cvr/cvw223.
Fujisawa T, Wang K, Niu X-L, Egginton S, Ahmad S, Hewett P, Kontos CD, Ahmed A. Angiopoietin-1 promotes atherosclerosis by increasing the proportion of circulating Gr1+ monocytes. Cardiovasc Res. 2017 Jan;113(1):81–89.
Journal cover image

Published In

Cardiovasc Res

DOI

EISSN

1755-3245

Publication Date

January 2017

Volume

113

Issue

1

Start / End Page

81 / 89

Location

England

Related Subject Headings

  • Vascular Endothelial Growth Factor A
  • Tissue Culture Techniques
  • Signal Transduction
  • Plaque, Atherosclerotic
  • Phenotype
  • Monocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Male
  • Humans