Skip to main content
Journal cover image

Three-Year Durability of Immune Responses Induced by HIV-DNA and HIV-Modified Vaccinia Virus Ankara and Effect of a Late HIV-Modified Vaccinia Virus Ankara Boost in Tanzanian Volunteers.

Publication ,  Journal Article
Joachim, A; Munseri, PJ; Nilsson, C; Bakari, M; Aboud, S; Lyamuya, EF; Tecleab, T; Liakina, V; Scarlatti, G; Robb, ML; Earl, PL; Moss, B ...
Published in: AIDS Res Hum Retroviruses
August 2017

We explored the duration of immune responses and the effect of a late third HIV-modified vaccinia virus Ankara (MVA) boost in HIV-DNA primed and HIV-MVA boosted Tanzanian volunteers. Twenty volunteers who had previously received three HIV-DNA and two HIV-MVA immunizations were given a third HIV-MVA immunization 3 years after the second HIV-MVA boost. At the time of the third HIV-MVA, 90% of the vaccinees had antibodies to HIV-1 subtype C gp140 (median titer 200) and 85% to subtype B gp160 (median titer 100). The majority of vaccinees had detectable antibody-dependent cellular cytotoxicity (ADCC)-mediating antibodies, 70% against CRF01_AE virus-infected cells (median titer 239) and 84% against CRF01_AE gp120-coated cells (median titer 499). A high proportion (74%) of vaccinees had IFN-γ ELISpot responses, 63% to Gag and 42% to Env, 3 years after the second HIV-MVA boost. After the third HIV-MVA, there was an increase in Env-binding antibodies and ADCC-mediating antibodies relative to the response seen at the time of the third HIV-MVA vaccination, p < .0001 and p < .05, respectively. The frequency of IFN-γ ELISpot responses increased to 95% against Gag or Env and 90% to both Gag and Env, p = .064 and p = .002, respectively. In conclusion, the HIV-DNA prime/HIV-MVA boost regimen elicited potent antibody and cellular immune responses with remarkable durability, and a third HIV-MVA immunization significantly boosted both antibody and cellular immune responses relative to the levels detected at the time of the third HIV-MVA, but not to higher levels than after the second HIV-MVA.

Duke Scholars

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

August 2017

Volume

33

Issue

8

Start / End Page

880 / 888

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Vaccines, Synthetic
  • Vaccines, DNA
  • Time Factors
  • Tanzania
  • Middle Aged
  • Male
  • Interferon-gamma
  • Immunization, Secondary
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Joachim, A., Munseri, P. J., Nilsson, C., Bakari, M., Aboud, S., Lyamuya, E. F., … Biberfeld, G. (2017). Three-Year Durability of Immune Responses Induced by HIV-DNA and HIV-Modified Vaccinia Virus Ankara and Effect of a Late HIV-Modified Vaccinia Virus Ankara Boost in Tanzanian Volunteers. AIDS Res Hum Retroviruses, 33(8), 880–888. https://doi.org/10.1089/AID.2016.0251
Joachim, Agricola, Patricia J. Munseri, Charlotta Nilsson, Muhammad Bakari, Said Aboud, Eligius F. Lyamuya, Teghesti Tecleab, et al. “Three-Year Durability of Immune Responses Induced by HIV-DNA and HIV-Modified Vaccinia Virus Ankara and Effect of a Late HIV-Modified Vaccinia Virus Ankara Boost in Tanzanian Volunteers.AIDS Res Hum Retroviruses 33, no. 8 (August 2017): 880–88. https://doi.org/10.1089/AID.2016.0251.
Joachim, Agricola, et al. “Three-Year Durability of Immune Responses Induced by HIV-DNA and HIV-Modified Vaccinia Virus Ankara and Effect of a Late HIV-Modified Vaccinia Virus Ankara Boost in Tanzanian Volunteers.AIDS Res Hum Retroviruses, vol. 33, no. 8, Aug. 2017, pp. 880–88. Pubmed, doi:10.1089/AID.2016.0251.
Joachim A, Munseri PJ, Nilsson C, Bakari M, Aboud S, Lyamuya EF, Tecleab T, Liakina V, Scarlatti G, Robb ML, Earl PL, Moss B, Wahren B, Mhalu F, Ferrari G, Sandstrom E, Biberfeld G. Three-Year Durability of Immune Responses Induced by HIV-DNA and HIV-Modified Vaccinia Virus Ankara and Effect of a Late HIV-Modified Vaccinia Virus Ankara Boost in Tanzanian Volunteers. AIDS Res Hum Retroviruses. 2017 Aug;33(8):880–888.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

August 2017

Volume

33

Issue

8

Start / End Page

880 / 888

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Vaccines, Synthetic
  • Vaccines, DNA
  • Time Factors
  • Tanzania
  • Middle Aged
  • Male
  • Interferon-gamma
  • Immunization, Secondary