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Wilms' tumor suppressor gene expression in rat and human mesothelioma.

Publication ,  Journal Article
Walker, C; Rutten, F; Yuan, X; Pass, H; Mew, DM; Everitt, J
Published in: Cancer Res
June 15, 1994

Induction of mesothelioma in the rat is an important animal model for assessing the carcinogenic potential of fibers and for understanding the molecular basis underlying the development of these tumors. Mesotheliomas and nephroblastoma (Wilms' tumor) have many developmental, biochemical, and histological similarities; however, the expression of the Wilms' tumor suppressor gene, WT-1, has not been well characterized in the rat, and its expression pattern in rat or human mesothelioma has not been described. We report that WT-1 transcripts (3.2 kilobases) could be detected by Northern analysis in adult rat testis, spleen, kidney, lung, heart, and glomerular mesangial cells. Normal adult mesothelial cells also expressed this gene. Rat mesothelioma cell lines expressed WT-1 transcripts of 3.2 kilobases and an additional 2.8-kilobase transcript, previously only reported to be expressed in the testis. Normal and transformed rat mesothelial cells expressed all four of the WT-1 splice variants, except testis, which only expressed WT-1 splice variants containing exon 5. Seven of seven human mesothelioma cell lines examined also expressed WT-1 transcripts, suggesting that expression of this gene may be useful in the diagnosis of these tumors.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1994

Volume

54

Issue

12

Start / End Page

3101 / 3106

Location

United States

Related Subject Headings

  • Testis
  • Rats
  • Organ Specificity
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Middle Aged
  • Mesothelioma
  • Male
  • Humans
  • Genes, Wilms Tumor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Walker, C., Rutten, F., Yuan, X., Pass, H., Mew, D. M., & Everitt, J. (1994). Wilms' tumor suppressor gene expression in rat and human mesothelioma. Cancer Res, 54(12), 3101–3106.
Walker, C., F. Rutten, X. Yuan, H. Pass, D. M. Mew, and J. Everitt. “Wilms' tumor suppressor gene expression in rat and human mesothelioma.Cancer Res 54, no. 12 (June 15, 1994): 3101–6.
Walker C, Rutten F, Yuan X, Pass H, Mew DM, Everitt J. Wilms' tumor suppressor gene expression in rat and human mesothelioma. Cancer Res. 1994 Jun 15;54(12):3101–6.
Walker, C., et al. “Wilms' tumor suppressor gene expression in rat and human mesothelioma.Cancer Res, vol. 54, no. 12, June 1994, pp. 3101–06.
Walker C, Rutten F, Yuan X, Pass H, Mew DM, Everitt J. Wilms' tumor suppressor gene expression in rat and human mesothelioma. Cancer Res. 1994 Jun 15;54(12):3101–3106.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1994

Volume

54

Issue

12

Start / End Page

3101 / 3106

Location

United States

Related Subject Headings

  • Testis
  • Rats
  • Organ Specificity
  • Oncology & Carcinogenesis
  • Molecular Sequence Data
  • Middle Aged
  • Mesothelioma
  • Male
  • Humans
  • Genes, Wilms Tumor