Skip to main content
Journal cover image

Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine.

Publication ,  Journal Article
Arthur, LO; Pyle, SW; Nara, PL; Bess, JW; Gonda, MA; Kelliher, JC; Gilden, RV; Robey, WG; Bolognesi, DP; Gallo, RC
Published in: Proc Natl Acad Sci U S A
December 1987

The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp120) was purified from membranes of human T-lymphotropic virus (HTLV)-IIIB-infected cells and the final preparation contained low levels to no detectable HTLV-IIIB core antigen (p24) and low levels of endotoxin. Chimpanzees inoculated with gp120 responded by developing antibodies that precipitated radiolabeled gp120 and neutralized in vitro infection of HTLV-IIIB. Antibodies to HTLV-IIIB p24 were not detected in the gp120-immunized chimpanzees. Peripheral blood leukocytes from the vaccinated animals were examined for T4+ and T8+ cells, and no decrease in the T4/T8 ratio was found, indicating that immunization with a ligand (gp120) that binds to T4 has no detectable adverse effect on the population of T4+ cells. The only current animal model that can be reproducibly infected with HIV is the chimpanzee. Immunization of chimpanzees with HIV proteins will provide an experimental system for testing the effectiveness of prototype vaccines for preventing HIV infection in vivo.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1987

Volume

84

Issue

23

Start / End Page

8583 / 8587

Location

United States

Related Subject Headings

  • Viral Vaccines
  • Viral Proteins
  • Pan troglodytes
  • Neutralization Tests
  • Molecular Weight
  • Immunization
  • HIV
  • Glycoproteins
  • Antigens, Viral
  • Antibodies, Viral
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Arthur, L. O., Pyle, S. W., Nara, P. L., Bess, J. W., Gonda, M. A., Kelliher, J. C., … Gallo, R. C. (1987). Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine. Proc Natl Acad Sci U S A, 84(23), 8583–8587. https://doi.org/10.1073/pnas.84.23.8583
Arthur, L. O., S. W. Pyle, P. L. Nara, J. W. Bess, M. A. Gonda, J. C. Kelliher, R. V. Gilden, W. G. Robey, D. P. Bolognesi, and R. C. Gallo. “Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine.Proc Natl Acad Sci U S A 84, no. 23 (December 1987): 8583–87. https://doi.org/10.1073/pnas.84.23.8583.
Arthur LO, Pyle SW, Nara PL, Bess JW, Gonda MA, Kelliher JC, et al. Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8583–7.
Arthur, L. O., et al. “Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine.Proc Natl Acad Sci U S A, vol. 84, no. 23, Dec. 1987, pp. 8583–87. Pubmed, doi:10.1073/pnas.84.23.8583.
Arthur LO, Pyle SW, Nara PL, Bess JW, Gonda MA, Kelliher JC, Gilden RV, Robey WG, Bolognesi DP, Gallo RC. Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8583–8587.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

December 1987

Volume

84

Issue

23

Start / End Page

8583 / 8587

Location

United States

Related Subject Headings

  • Viral Vaccines
  • Viral Proteins
  • Pan troglodytes
  • Neutralization Tests
  • Molecular Weight
  • Immunization
  • HIV
  • Glycoproteins
  • Antigens, Viral
  • Antibodies, Viral