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Role of carbohydrate in biological functions of Friend murine leukemia virus gp71.

Publication ,  Journal Article
Schäfer, W; Fischinger, PJ; Collins, JJ; Bolognesi, DP
Published in: J Virol
January 1977

Purified gp71 of Friend murine leukemia virus (FLV) can interfere with virus infection, absorb neutralizing antibody, and in the presence of group-specific anti-gp71 antibody, hemagglutinate sheep erythrocytes. Interference by FLV gp71 with several murine leukemia viruses (MuLV) was tested in the XC and S + L- assay systems. Treatment of gp71 with trypsin or Pronase eliminated its interfering capacity. However, treatment with neuraminidase or a mixture of glycosidase enzymes, which left the major serological properties of gp71 intact, did not reduce the interference potential of gp71 for FLV or AKR MuLV. The capacity of gp71 to absorb type- or group-specific virus-neutralizing antibodies was similarly affected by the various enzyme treatments. In contrast, indirect hemagglutination by gp71 was abolished not only by proteases but also by treatment with glycosidase enzymes, although neuraminidase had no effect. Preliminary data indicate that infectivity of FLV or xenotropic MuLV was not affected by short treatment with glycosidase enzymes.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1977

Volume

21

Issue

1

Start / End Page

35 / 40

Location

United States

Related Subject Headings

  • Virology
  • Viral Interference
  • Trypsin
  • Pronase
  • Neuraminidase
  • Hemagglutination, Viral
  • Glycoside Hydrolases
  • Friend murine leukemia virus
  • Carbohydrates
  • Carbohydrate Metabolism
 

Citation

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MLA
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Schäfer, W., Fischinger, P. J., Collins, J. J., & Bolognesi, D. P. (1977). Role of carbohydrate in biological functions of Friend murine leukemia virus gp71. J Virol, 21(1), 35–40. https://doi.org/10.1128/JVI.21.1.35-40.1977
Schäfer, W., P. J. Fischinger, J. J. Collins, and D. P. Bolognesi. “Role of carbohydrate in biological functions of Friend murine leukemia virus gp71.J Virol 21, no. 1 (January 1977): 35–40. https://doi.org/10.1128/JVI.21.1.35-40.1977.
Schäfer W, Fischinger PJ, Collins JJ, Bolognesi DP. Role of carbohydrate in biological functions of Friend murine leukemia virus gp71. J Virol. 1977 Jan;21(1):35–40.
Schäfer, W., et al. “Role of carbohydrate in biological functions of Friend murine leukemia virus gp71.J Virol, vol. 21, no. 1, Jan. 1977, pp. 35–40. Pubmed, doi:10.1128/JVI.21.1.35-40.1977.
Schäfer W, Fischinger PJ, Collins JJ, Bolognesi DP. Role of carbohydrate in biological functions of Friend murine leukemia virus gp71. J Virol. 1977 Jan;21(1):35–40.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1977

Volume

21

Issue

1

Start / End Page

35 / 40

Location

United States

Related Subject Headings

  • Virology
  • Viral Interference
  • Trypsin
  • Pronase
  • Neuraminidase
  • Hemagglutination, Viral
  • Glycoside Hydrolases
  • Friend murine leukemia virus
  • Carbohydrates
  • Carbohydrate Metabolism