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Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus.

Publication ,  Journal Article
Bolognesi, DP; Collins, JJ; Leis, JP; Moennig, V; Schäfer, W; Atkinson, PH
Published in: J Virol
December 1975

Treatment of Friend leukemia virus gp71 with protease-free glycosidase enzymes results in removal of the major portion of the carbohydrate without affecting the amount of protein present. The digested material migrates as protein of about 60,000 to 65,000 molecular weight on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Analyses of the serological properties of gp71 after enzyme treatment indicated that the type, group, and interspecies determinants were not destroyed. In contrast, treatment with proteolytic enzymes led to the complete destruction of the gp71 molecule, including the total elimination of its serological reactivity as measured by direct and competition radioimmunoassay and by a serum cytotoxicity assay. We conclude that the carbohydrate portion of gp71 is not of major significance in defining the antigenic determinants of this viral glycoprotein.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1975

Volume

16

Issue

6

Start / End Page

1453 / 1463

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Radioimmunoassay
  • Pronase
  • Neuraminidase
  • Molecular Weight
  • Glycoside Hydrolases
  • Glycoproteins
  • Friend murine leukemia virus
  • Epitopes
 

Citation

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MLA
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Bolognesi, D. P., Collins, J. J., Leis, J. P., Moennig, V., Schäfer, W., & Atkinson, P. H. (1975). Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus. J Virol, 16(6), 1453–1463. https://doi.org/10.1128/JVI.16.6.1453-1463.1975
Bolognesi, D. P., J. J. Collins, J. P. Leis, V. Moennig, W. Schäfer, and P. H. Atkinson. “Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus.J Virol 16, no. 6 (December 1975): 1453–63. https://doi.org/10.1128/JVI.16.6.1453-1463.1975.
Bolognesi DP, Collins JJ, Leis JP, Moennig V, Schäfer W, Atkinson PH. Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus. J Virol. 1975 Dec;16(6):1453–63.
Bolognesi, D. P., et al. “Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus.J Virol, vol. 16, no. 6, Dec. 1975, pp. 1453–63. Pubmed, doi:10.1128/JVI.16.6.1453-1463.1975.
Bolognesi DP, Collins JJ, Leis JP, Moennig V, Schäfer W, Atkinson PH. Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus. J Virol. 1975 Dec;16(6):1453–1463.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

December 1975

Volume

16

Issue

6

Start / End Page

1453 / 1463

Location

United States

Related Subject Headings

  • Virology
  • Viral Proteins
  • Radioimmunoassay
  • Pronase
  • Neuraminidase
  • Molecular Weight
  • Glycoside Hydrolases
  • Glycoproteins
  • Friend murine leukemia virus
  • Epitopes