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Reactivity of avian RNA tumor viruses with lectins.

Publication ,  Journal Article
Ishizaki, R; Bolognesi, DP
Published in: J Virol
January 1975

The infectivity of avian RNA tumor viruses was inactivated to varying degrees by treatment with either concanavalin A (Con A) or phytohemagglutinin but not by treatment with wheat germ agglutinin. In general, leukosis viruses reacted preferentially with Con A, whereas sarcoma viruses showed more affinity for phytohemagglutinin. In a more extensive study with subgroup A of Prague Rous sarcoma virus (PR-A), the effect of inactivation by Con A could be specifically prevented by the addition of alpha-methyl-D-mannoside, alpha-methyl-D-glucoside, and N-acetyl-D-glucosamine. These sugars were also capable of eluting [3H]glucosamine-labeled material from disrupted PR-A virus, which was bound to a Con A-sepharose affinity column. A major viral glycoprotein recovered from the column had the same mobility as gp85 in polyacrylamide gel electrophoresis and could be immunoprecipitated with anti-gp85 antiserum. These results suggest that the material reacting with Con A is present on the gp85 component of the viral glycoprotein. The diversity in the reactivity of the glycoproteins of transforming and nontransforming viruses with plant lectins is discussed.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1975

Volume

17

Issue

1

Start / End Page

132 / 139

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Vegetables
  • Triticum
  • Plant Lectins
  • Methylmannosides
  • Methylglucosides
  • Lectins
  • Glycoproteins
 

Citation

APA
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ICMJE
MLA
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Ishizaki, R., & Bolognesi, D. P. (1975). Reactivity of avian RNA tumor viruses with lectins. J Virol, 17(1), 132–139. https://doi.org/10.1128/JVI.17.1.132-139.1976
Ishizaki, R., and D. P. Bolognesi. “Reactivity of avian RNA tumor viruses with lectins.J Virol 17, no. 1 (January 1975): 132–39. https://doi.org/10.1128/JVI.17.1.132-139.1976.
Ishizaki R, Bolognesi DP. Reactivity of avian RNA tumor viruses with lectins. J Virol. 1975 Jan;17(1):132–9.
Ishizaki, R., and D. P. Bolognesi. “Reactivity of avian RNA tumor viruses with lectins.J Virol, vol. 17, no. 1, Jan. 1975, pp. 132–39. Pubmed, doi:10.1128/JVI.17.1.132-139.1976.
Ishizaki R, Bolognesi DP. Reactivity of avian RNA tumor viruses with lectins. J Virol. 1975 Jan;17(1):132–139.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

January 1975

Volume

17

Issue

1

Start / End Page

132 / 139

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Vegetables
  • Triticum
  • Plant Lectins
  • Methylmannosides
  • Methylglucosides
  • Lectins
  • Glycoproteins