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Protein unfolding under isometric tension-what force can integrins generate, and can it unfold FNIII domains?

Publication ,  Journal Article
Erickson, HP
Published in: Curr Opin Struct Biol
February 2017

Extracellular matrix fibrils of fibronectin (FN) are highly elastic, and are typically stretched three to four times their relaxed length. The mechanism of stretching has been controversial, in particular whether it involves tension-induced unfolding of FNIII domains. Recent studies have found that ∼5pN is the threshold isometric force for unfolding various protein domains. FNIII domains should therefore not be unfolded until the tension approaches 5pN. Integrins have been reported to generate forces ranging from 1 to >50pN, but I argue that studies reporting 1-2pN are the most convincing. This is not enough to unfold FNIII domains. Even if domains were unfolded, 2pN would only extend the worm-like-chain to about twice the length of the folded domain. Overall I conclude that stretching FN matrix fibrils involves primarily the compact to extended conformational change of FN dimers, with minimal contribution from unfolding FNIII domains.

Duke Scholars

Published In

Curr Opin Struct Biol

DOI

EISSN

1879-033X

Publication Date

February 2017

Volume

42

Start / End Page

98 / 105

Location

England

Related Subject Headings

  • Protein Unfolding
  • Protein Refolding
  • Protein Domains
  • Mechanical Phenomena
  • Integrins
  • Fibronectins
  • Biophysics
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry
 

Citation

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Erickson, H. P. (2017). Protein unfolding under isometric tension-what force can integrins generate, and can it unfold FNIII domains? Curr Opin Struct Biol, 42, 98–105. https://doi.org/10.1016/j.sbi.2016.12.002
Erickson, Harold P. “Protein unfolding under isometric tension-what force can integrins generate, and can it unfold FNIII domains?Curr Opin Struct Biol 42 (February 2017): 98–105. https://doi.org/10.1016/j.sbi.2016.12.002.
Erickson, Harold P. “Protein unfolding under isometric tension-what force can integrins generate, and can it unfold FNIII domains?Curr Opin Struct Biol, vol. 42, Feb. 2017, pp. 98–105. Pubmed, doi:10.1016/j.sbi.2016.12.002.
Journal cover image

Published In

Curr Opin Struct Biol

DOI

EISSN

1879-033X

Publication Date

February 2017

Volume

42

Start / End Page

98 / 105

Location

England

Related Subject Headings

  • Protein Unfolding
  • Protein Refolding
  • Protein Domains
  • Mechanical Phenomena
  • Integrins
  • Fibronectins
  • Biophysics
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
  • 0304 Medicinal and Biomolecular Chemistry