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Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST.

Publication ,  Journal Article
Ritchie, JC; Owens, MJ; Mayer, H; Watson, JT; Kilts, C; Carroll, BJ
Published in: Biol Psychiatry
November 1, 1992

The role of the metabolites of dexamethasone (DEX) in the dexamethasone suppression test (DST) has never been fully elucidated. We report here our preliminary studies of 6 beta-hydroxydexamethasone (6 OH-Dex), a known metabolite of DEX, on the hypothalamic-pituitary-adrenal (HPA) axis of the rat; its activity in the most commonly used radioimmunoassay for plasma DEX; and its plasma concentrations in a normal human subject during the standard 1.0 mg DST. Six OH-Dex administered subcutaneously to rats at a dose of 1 mg/kg was able to completely suppress corticosterone production for at least 3 hr. In the IgG Corp. radioimmunoassay for plasma DEX, 6 OH-Dex was moderately cross-reactive yielding a 50% cross-reactivity of 10%. Gas chromatographic coupled mass spectroscopic analysis of human plasma samples, obtained 12 to 20 hr after the oral ingestion of 1.0 mg DEX, demonstrated similar plasma concentrations for both the parent compound and the 6-hydroxyl metabolite. The relevance of these findings, particularly to pharmacokinetic studies of the DST, is discussed.

Published In

Biol Psychiatry

DOI

ISSN

0006-3223

Publication Date

November 1, 1992

Volume

32

Issue

9

Start / End Page

825 / 833

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Radioimmunoassay
  • Psychiatry
  • Male
  • Hydrocortisone
  • Gas Chromatography-Mass Spectrometry
  • Dexamethasone
  • Corticosterone
  • Animals
 

Citation

APA
Chicago
ICMJE
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Ritchie, J. C., Owens, M. J., Mayer, H., Watson, J. T., Kilts, C., & Carroll, B. J. (1992). Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST. Biol Psychiatry, 32(9), 825–833. https://doi.org/10.1016/0006-3223(92)90086-f
Ritchie, J. C., M. J. Owens, H. Mayer, J. T. Watson, C. Kilts, and B. J. Carroll. “Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST.Biol Psychiatry 32, no. 9 (November 1, 1992): 825–33. https://doi.org/10.1016/0006-3223(92)90086-f.
Ritchie JC, Owens MJ, Mayer H, Watson JT, Kilts C, Carroll BJ. Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST. Biol Psychiatry. 1992 Nov 1;32(9):825–33.
Ritchie, J. C., et al. “Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST.Biol Psychiatry, vol. 32, no. 9, Nov. 1992, pp. 825–33. Pubmed, doi:10.1016/0006-3223(92)90086-f.
Ritchie JC, Owens MJ, Mayer H, Watson JT, Kilts C, Carroll BJ. Preliminary studies of 6 beta-hydroxydexamethasone and its importance in the DST. Biol Psychiatry. 1992 Nov 1;32(9):825–833.
Journal cover image

Published In

Biol Psychiatry

DOI

ISSN

0006-3223

Publication Date

November 1, 1992

Volume

32

Issue

9

Start / End Page

825 / 833

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Radioimmunoassay
  • Psychiatry
  • Male
  • Hydrocortisone
  • Gas Chromatography-Mass Spectrometry
  • Dexamethasone
  • Corticosterone
  • Animals