Skip to main content
Journal cover image

The effect of serial dilution error on calibration inference in immunoassay.

Publication ,  Journal Article
Higgins, KM; Davidian, M; Chew, G; Burge, H
Published in: Biometrics
March 1998

A common practice in immunoassay is the use of sequential dilutions of an initial stock solution of the antigen of interest to obtain standard samples in a desired concentration range. Nonlinear, heteroscedastic regression models are a common framework for analysis, and the usual methods for fitting the model assume that measured responses on the standards are independent. However, the dilution procedure introduces a propagation of random measurement error that may invalidate this assumption. We demonstrate that failure to account for serial dilution error in calibration inference on unknown samples leads to serious inaccuracy of assessments of assay precision such as confidence intervals and precision profiles. Techniques for taking serial dilution error into account based on data from multiple assay runs are discussed and are shown to yield valid calibration inferences.

Duke Scholars

Published In

Biometrics

ISSN

0006-341X

Publication Date

March 1998

Volume

54

Issue

1

Start / End Page

19 / 32

Location

England

Related Subject Headings

  • Statistics & Probability
  • Reference Standards
  • Radioimmunoassay
  • Monte Carlo Method
  • Models, Statistical
  • Mites
  • Immunoassay
  • Humans
  • Glycoproteins
  • Enzyme-Linked Immunosorbent Assay
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Higgins, K. M., Davidian, M., Chew, G., & Burge, H. (1998). The effect of serial dilution error on calibration inference in immunoassay. Biometrics, 54(1), 19–32.
Higgins, K. M., M. Davidian, G. Chew, and H. Burge. “The effect of serial dilution error on calibration inference in immunoassay.Biometrics 54, no. 1 (March 1998): 19–32.
Higgins KM, Davidian M, Chew G, Burge H. The effect of serial dilution error on calibration inference in immunoassay. Biometrics. 1998 Mar;54(1):19–32.
Higgins, K. M., et al. “The effect of serial dilution error on calibration inference in immunoassay.Biometrics, vol. 54, no. 1, Mar. 1998, pp. 19–32.
Higgins KM, Davidian M, Chew G, Burge H. The effect of serial dilution error on calibration inference in immunoassay. Biometrics. 1998 Mar;54(1):19–32.
Journal cover image

Published In

Biometrics

ISSN

0006-341X

Publication Date

March 1998

Volume

54

Issue

1

Start / End Page

19 / 32

Location

England

Related Subject Headings

  • Statistics & Probability
  • Reference Standards
  • Radioimmunoassay
  • Monte Carlo Method
  • Models, Statistical
  • Mites
  • Immunoassay
  • Humans
  • Glycoproteins
  • Enzyme-Linked Immunosorbent Assay