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Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.

Publication ,  Journal Article
Farr, AK; Braun, RD; Cefalu, WT; Bell-Farrow, AD; Wang, ZQ; Hatchell, DL
Published in: Lab Anim Sci
February 1999

One of the earliest pathologic changes of diabetes mellitus is increased nonenzymatic glycosylation (i.e., glycation) of proteins, which results in abnormal aggregation of collagen fibrils and production of superoxide radicals. These abnormalities may be responsible for the precocious senescence of connective tissue associated with the disease. We sought to determine whether glycation is increased in the vitreous humor of short-term diabetic cats (6 months' duration) and rabbits (2 months' duration), using a nitroblue tetrazolium colorimetric assay for fructosamine. Vitreous protein fructosamine concentration was significantly higher in diabetic cats and rabbits, compared with that in control (nondiabetic) animals. These results indicate that glycation is increased in the vitreous humor of short-term diabetic animals, and therefore may be one of the initial triggers for clinically apparent diabetic retinopathy.

Duke Scholars

Published In

Lab Anim Sci

ISSN

0023-6764

Publication Date

February 1999

Volume

49

Issue

1

Start / End Page

58 / 61

Location

United States

Related Subject Headings

  • Vitreous Body
  • Veterinary Sciences
  • Rabbits
  • Pancreatectomy
  • Glycosylation
  • Glycoproteins
  • Glycated Hemoglobin
  • Glucose
  • Fructosamine
  • Diabetes Mellitus, Experimental
 

Citation

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MLA
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Farr, A. K., Braun, R. D., Cefalu, W. T., Bell-Farrow, A. D., Wang, Z. Q., & Hatchell, D. L. (1999). Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals. Lab Anim Sci, 49(1), 58–61.
Farr, A. K., R. D. Braun, W. T. Cefalu, A. D. Bell-Farrow, Z. Q. Wang, and D. L. Hatchell. “Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.Lab Anim Sci 49, no. 1 (February 1999): 58–61.
Farr AK, Braun RD, Cefalu WT, Bell-Farrow AD, Wang ZQ, Hatchell DL. Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals. Lab Anim Sci. 1999 Feb;49(1):58–61.
Farr, A. K., et al. “Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals.Lab Anim Sci, vol. 49, no. 1, Feb. 1999, pp. 58–61.
Farr AK, Braun RD, Cefalu WT, Bell-Farrow AD, Wang ZQ, Hatchell DL. Increased nonenzymatically glycosylated proteins in the vitreous humor of diabetic animals. Lab Anim Sci. 1999 Feb;49(1):58–61.

Published In

Lab Anim Sci

ISSN

0023-6764

Publication Date

February 1999

Volume

49

Issue

1

Start / End Page

58 / 61

Location

United States

Related Subject Headings

  • Vitreous Body
  • Veterinary Sciences
  • Rabbits
  • Pancreatectomy
  • Glycosylation
  • Glycoproteins
  • Glycated Hemoglobin
  • Glucose
  • Fructosamine
  • Diabetes Mellitus, Experimental