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HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies.

Publication ,  Journal Article
Meyerhoff, RR; Scearce, RM; Ogburn, DF; Lockwood, B; Pickeral, J; Kuraoka, M; Anasti, K; Eudailey, J; Eaton, A; Cooper, M; Wiehe, K; Alam, SM ...
Published in: AIDS Res Hum Retroviruses
August 2017

Antibodies that cross-react with multiple HIV-1 envelopes (Envs) are useful reagents for characterizing Env proteins and for soluble Env capture and purification assays. We previously reported 10 murine monoclonal antibodies induced by group M consensus Env, CON-6 immunization. Each demonstrated broad cross-reactivity to recombinant Envs. Here we characterized the Env epitopes to which they bind. Seven mapped to linear epitopes in gp120, five at the Env N-terminus, and two at the Env C-terminus. One antibody, 13D7, bound at the gp120 N-terminus (aa 30-42), reacted with HIV-1-infected CD4+ T cells, and when expressed in a human IgG1 backbone, mediated antibody-dependent cellular cytotoxicity. Antibody 18F11 bound at the gp120 C-terminus (aa 445-459) and reactivity was glycan dependent. Antibodies 13D7, 3B3, and 16H3 bound to 100 percent of HIV-1 Envs tested in ELISA and sodium dodecyl sulfate/polyacrylamide gel electrophoresis/western blot analysis. These data define the epitopes of monoclonal antibody reagents for characterization of recombinant Envs, one epitope of which is also expressed on the surface of HIV-1-infected CD4+ T cells.

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Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

August 2017

Volume

33

Issue

8

Start / End Page

859 / 868

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Mice, Inbred BALB C
  • HIV-1
  • HIV Antibodies
  • Epitopes
  • Epitope Mapping
  • Consensus Sequence
  • Antibody-Dependent Cell Cytotoxicity
  • Antibodies, Monoclonal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Meyerhoff, R. R., Scearce, R. M., Ogburn, D. F., Lockwood, B., Pickeral, J., Kuraoka, M., … Haynes, B. F. (2017). HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies. AIDS Res Hum Retroviruses, 33(8), 859–868. https://doi.org/10.1089/AID.2016.0294
Meyerhoff, R Ryan, Richard M. Scearce, Damon F. Ogburn, Brad Lockwood, Joy Pickeral, Masa Kuraoka, Kara Anasti, et al. “HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies.AIDS Res Hum Retroviruses 33, no. 8 (August 2017): 859–68. https://doi.org/10.1089/AID.2016.0294.
Meyerhoff RR, Scearce RM, Ogburn DF, Lockwood B, Pickeral J, Kuraoka M, et al. HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies. AIDS Res Hum Retroviruses. 2017 Aug;33(8):859–68.
Meyerhoff, R. Ryan, et al. “HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies.AIDS Res Hum Retroviruses, vol. 33, no. 8, Aug. 2017, pp. 859–68. Pubmed, doi:10.1089/AID.2016.0294.
Meyerhoff RR, Scearce RM, Ogburn DF, Lockwood B, Pickeral J, Kuraoka M, Anasti K, Eudailey J, Eaton A, Cooper M, Wiehe K, Montefiori DC, Tomaras G, Ferrari G, Alam SM, Liao H-X, Korber B, Gao F, Haynes BF. HIV-1 Consensus Envelope-Induced Broadly Binding Antibodies. AIDS Res Hum Retroviruses. 2017 Aug;33(8):859–868.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

EISSN

1931-8405

Publication Date

August 2017

Volume

33

Issue

8

Start / End Page

859 / 868

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Mice, Inbred BALB C
  • HIV-1
  • HIV Antibodies
  • Epitopes
  • Epitope Mapping
  • Consensus Sequence
  • Antibody-Dependent Cell Cytotoxicity
  • Antibodies, Monoclonal