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Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies.

Publication ,  Journal Article
Jiranek, W; Jasty, M; Wang, JT; Bragdon, C; Wolfe, H; Goldberg, M; Harris, W
Published in: J Bone Joint Surg Am
November 1995

We examined the tissue response to subcutaneous injections of particulate polymethylmethacrylate powder in fully immunocompetent C3Hf/Sed mice as well as three strains of mice with different levels of lymphocyte dysfunction. Five weeks after the injection, we found clearly demarcated granulomas. Histological and immunohistochemical studies showed that these granulomas were similar among all strains, with either paucity or absence of lymphoid cells. In situ hybridization with use of complementary RNA probes indicated that macrophages were synthesizing interleukin-1 beta messenger RNA (mRNA), a marker of macrophage activation, and a cytokine implicated in pathological bone resorption. We concluded that, in mice, there is a lymphocyte-independent pathway of macrophage activation in response to particulate polymethylmethacrylate. This suggests that the foreign-body response to particulate orthopaedic biomaterials is macrophage-initiated and maintained and that lymphocytes are not essential to this response, although they may modulate it.

Duke Scholars

Published In

J Bone Joint Surg Am

DOI

ISSN

0021-9355

Publication Date

November 1995

Volume

77

Issue

11

Start / End Page

1650 / 1661

Location

United States

Related Subject Headings

  • Particle Size
  • Orthopedics
  • Mice, SCID
  • Mice, Nude
  • Mice, Inbred C3H
  • Mice
  • Methylmethacrylates
  • Macrophages
  • Macrophage Activation
  • Lymphocytes
 

Citation

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Jiranek, W., Jasty, M., Wang, J. T., Bragdon, C., Wolfe, H., Goldberg, M., & Harris, W. (1995). Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies. J Bone Joint Surg Am, 77(11), 1650–1661. https://doi.org/10.2106/00004623-199511000-00003
Jiranek, W., M. Jasty, J. T. Wang, C. Bragdon, H. Wolfe, M. Goldberg, and W. Harris. “Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies.J Bone Joint Surg Am 77, no. 11 (November 1995): 1650–61. https://doi.org/10.2106/00004623-199511000-00003.
Jiranek W, Jasty M, Wang JT, Bragdon C, Wolfe H, Goldberg M, et al. Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies. J Bone Joint Surg Am. 1995 Nov;77(11):1650–61.
Jiranek, W., et al. “Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies.J Bone Joint Surg Am, vol. 77, no. 11, Nov. 1995, pp. 1650–61. Pubmed, doi:10.2106/00004623-199511000-00003.
Jiranek W, Jasty M, Wang JT, Bragdon C, Wolfe H, Goldberg M, Harris W. Tissue response to particulate polymethylmethacrylate in mice with various immune deficiencies. J Bone Joint Surg Am. 1995 Nov;77(11):1650–1661.
Journal cover image

Published In

J Bone Joint Surg Am

DOI

ISSN

0021-9355

Publication Date

November 1995

Volume

77

Issue

11

Start / End Page

1650 / 1661

Location

United States

Related Subject Headings

  • Particle Size
  • Orthopedics
  • Mice, SCID
  • Mice, Nude
  • Mice, Inbred C3H
  • Mice
  • Methylmethacrylates
  • Macrophages
  • Macrophage Activation
  • Lymphocytes