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Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization.

Publication ,  Journal Article
Jiranek, WA; Machado, M; Jasty, M; Jevsevar, D; Wolfe, HJ; Goldring, SR; Goldberg, MJ; Harris, WH
Published in: J Bone Joint Surg Am
June 1993

The chronic inflammatory response to wear particles from orthopaedic joint implants is believed to cause osteolysis and to contribute to prosthetic loosening. Previous in vitro experiments have demonstrated that particulate debris from joint implants causes cells in culture to release products that have been implicated in this pathological bone resorption. The purpose of the current study was to investigate the in vivo features of this complex process in patients who had had a total hip replacement. Membraneous tissue was obtained from the cement-bone interface of ten polyethylene acetabular components that had been revised for aseptic loosening in ten patients. The immunoperoxidase technique, which involves the use of specific antibodies for each cell type, showed that macrophages were the predominant cellular constituents but also that fibroblasts, many of which were not identified on plain histological study, were present and were actively producing collagen. T lymphocytes were present variably, but they generally composed less than 10 percent of the cells. Particulate debris (polyethylene, methylmethacrylate, and metal) was present in all membrane specimens but was intracellular only in macrophages and multinucleated giant cells. 35S-labeled nucleic-acid probes, complementary to human interleukin-1-beta and to platelet-derived growth-factor-2 messenger RNA (mRNA), were hybridized with serial tissue sections. Hybridization demonstrated interleukin-1-beta mRNA predominantly in macrophages, and not in fibroblasts or in T lymphocytes to any major extent. In contrast, immunolocalization demonstrated interleukin-1-beta protein on both macrophages and fibroblasts, suggesting that macrophages release interleukin-1-beta, which then binds to both fibroblasts and macrophages. Platelet-derived growth-factor transcripts were found in both macrophages and fibroblasts.

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Published In

J Bone Joint Surg Am

DOI

ISSN

0021-9355

Publication Date

June 1993

Volume

75

Issue

6

Start / End Page

863 / 879

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Prosthesis Failure
  • Platelet-Derived Growth Factor
  • Orthopedics
  • Middle Aged
  • Male
  • Macrophages
  • Lymphocytes
  • Interleukin-1
  • In Situ Hybridization
 

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Jiranek, W. A., Machado, M., Jasty, M., Jevsevar, D., Wolfe, H. J., Goldring, S. R., … Harris, W. H. (1993). Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization. J Bone Joint Surg Am, 75(6), 863–879. https://doi.org/10.2106/00004623-199306000-00007
Jiranek, W. A., M. Machado, M. Jasty, D. Jevsevar, H. J. Wolfe, S. R. Goldring, M. J. Goldberg, and W. H. Harris. “Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization.J Bone Joint Surg Am 75, no. 6 (June 1993): 863–79. https://doi.org/10.2106/00004623-199306000-00007.
Jiranek WA, Machado M, Jasty M, Jevsevar D, Wolfe HJ, Goldring SR, et al. Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization. J Bone Joint Surg Am. 1993 Jun;75(6):863–79.
Jiranek, W. A., et al. “Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization.J Bone Joint Surg Am, vol. 75, no. 6, June 1993, pp. 863–79. Pubmed, doi:10.2106/00004623-199306000-00007.
Jiranek WA, Machado M, Jasty M, Jevsevar D, Wolfe HJ, Goldring SR, Goldberg MJ, Harris WH. Production of cytokines around loosened cemented acetabular components. Analysis with immunohistochemical techniques and in situ hybridization. J Bone Joint Surg Am. 1993 Jun;75(6):863–879.
Journal cover image

Published In

J Bone Joint Surg Am

DOI

ISSN

0021-9355

Publication Date

June 1993

Volume

75

Issue

6

Start / End Page

863 / 879

Location

United States

Related Subject Headings

  • RNA, Messenger
  • Prosthesis Failure
  • Platelet-Derived Growth Factor
  • Orthopedics
  • Middle Aged
  • Male
  • Macrophages
  • Lymphocytes
  • Interleukin-1
  • In Situ Hybridization