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Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A.

Publication ,  Journal Article
Blackburn, SD; Cline, SE; Hemming, JP; Johnson, FB
Published in: Arch Virol
July 2005

The bovine parvovirus (BPV) hemagglutinates human erythrocytes by binding to glycophorin A (GPA). The purpose of this study was to determine which carbohydrate on GPA binds BPV. Treatment of GPA with alpha2,3,-6,-8 neuraminidase eliminated binding of BPV to GPA. Beta-elimination of O-linked sialic acids on GPA eliminated binding, while removal of N-linked carbohydrates using the N-glycosidase PNGase F failed to eliminate binding. Treatment of GPA with a neuraminidase which specifically cleaved alpha2,3 glycosidic bonds eliminated BPV binding and, following this treatment, virus binding to GPA was restored by reconstitution of alpha2,3-linked neuraminic acids. These results indicated the O-linked alpha2,3 neuraminic acids of GPA bind BPV.

Duke Scholars

Published In

Arch Virol

DOI

ISSN

0304-8608

Publication Date

July 2005

Volume

150

Issue

7

Start / End Page

1477 / 1484

Location

Austria

Related Subject Headings

  • Virology
  • Receptors, Virus
  • Parvovirus
  • Orthomyxoviridae
  • N-Acetylneuraminic Acid
  • Hemagglutination
  • Glycophorins
  • Cell Membrane
  • Cattle
  • Animals
 

Citation

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Blackburn, S. D., Cline, S. E., Hemming, J. P., & Johnson, F. B. (2005). Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A. Arch Virol, 150(7), 1477–1484. https://doi.org/10.1007/s00705-005-0496-y
Blackburn, S. D., S. E. Cline, J. P. Hemming, and F. B. Johnson. “Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A.Arch Virol 150, no. 7 (July 2005): 1477–84. https://doi.org/10.1007/s00705-005-0496-y.
Blackburn SD, Cline SE, Hemming JP, Johnson FB. Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A. Arch Virol. 2005 Jul;150(7):1477–84.
Blackburn, S. D., et al. “Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A.Arch Virol, vol. 150, no. 7, July 2005, pp. 1477–84. Pubmed, doi:10.1007/s00705-005-0496-y.
Blackburn SD, Cline SE, Hemming JP, Johnson FB. Attachment of bovine parvovirus to O-linked alpha 2,3 neuraminic acid on glycophorin A. Arch Virol. 2005 Jul;150(7):1477–1484.
Journal cover image

Published In

Arch Virol

DOI

ISSN

0304-8608

Publication Date

July 2005

Volume

150

Issue

7

Start / End Page

1477 / 1484

Location

Austria

Related Subject Headings

  • Virology
  • Receptors, Virus
  • Parvovirus
  • Orthomyxoviridae
  • N-Acetylneuraminic Acid
  • Hemagglutination
  • Glycophorins
  • Cell Membrane
  • Cattle
  • Animals