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In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.

Publication ,  Journal Article
Mitchell, NS; Hagel, L; Fernandez, EJ
Published in: J Chromatogr A
August 29, 1997

Magnetic resonance imaging has been used to visualize size-based protein separations inside operating chromatography columns. The effects of flow nonuniformity have been observed and analyzed quantitatively through concentration profiles of tracers measured inside the column. Analysis of these profiles provides local and averaged intracolumn plate height values for characterization of dispersion and flow nonuniformity. The magnetic resonance measurements compare favorably with conventional chromatographic measurements of column efficiency and provide more detailed insights into nonideal column performance.

Duke Scholars

Published In

J Chromatogr A

DOI

ISSN

0021-9673

Publication Date

August 29, 1997

Volume

779

Issue

1-2

Start / End Page

73 / 89

Location

Netherlands

Related Subject Headings

  • Serum Albumin
  • Osmolar Concentration
  • Magnetic Resonance Imaging
  • Gadolinium DTPA
  • Chymotrypsinogen
  • Chromatography, Gel
  • Cattle
  • Animals
  • Analytical Chemistry
  • 40 Engineering
 

Citation

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Mitchell, N. S., Hagel, L., & Fernandez, E. J. (1997). In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A, 779(1–2), 73–89. https://doi.org/10.1016/s0021-9673(97)00457-3
Mitchell, N. S., L. Hagel, and E. J. Fernandez. “In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.J Chromatogr A 779, no. 1–2 (August 29, 1997): 73–89. https://doi.org/10.1016/s0021-9673(97)00457-3.
Mitchell NS, Hagel L, Fernandez EJ. In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A. 1997 Aug 29;779(1–2):73–89.
Mitchell, N. S., et al. “In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.J Chromatogr A, vol. 779, no. 1–2, Aug. 1997, pp. 73–89. Pubmed, doi:10.1016/s0021-9673(97)00457-3.
Mitchell NS, Hagel L, Fernandez EJ. In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A. 1997 Aug 29;779(1–2):73–89.
Journal cover image

Published In

J Chromatogr A

DOI

ISSN

0021-9673

Publication Date

August 29, 1997

Volume

779

Issue

1-2

Start / End Page

73 / 89

Location

Netherlands

Related Subject Headings

  • Serum Albumin
  • Osmolar Concentration
  • Magnetic Resonance Imaging
  • Gadolinium DTPA
  • Chymotrypsinogen
  • Chromatography, Gel
  • Cattle
  • Animals
  • Analytical Chemistry
  • 40 Engineering