In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.
Publication
, Journal Article
Mitchell, NS; Hagel, L; Fernandez, EJ
Published in: J Chromatogr A
August 29, 1997
Magnetic resonance imaging has been used to visualize size-based protein separations inside operating chromatography columns. The effects of flow nonuniformity have been observed and analyzed quantitatively through concentration profiles of tracers measured inside the column. Analysis of these profiles provides local and averaged intracolumn plate height values for characterization of dispersion and flow nonuniformity. The magnetic resonance measurements compare favorably with conventional chromatographic measurements of column efficiency and provide more detailed insights into nonideal column performance.
Duke Scholars
Published In
J Chromatogr A
DOI
ISSN
0021-9673
Publication Date
August 29, 1997
Volume
779
Issue
1-2
Start / End Page
73 / 89
Location
Netherlands
Related Subject Headings
- Serum Albumin
- Osmolar Concentration
- Magnetic Resonance Imaging
- Gadolinium DTPA
- Chymotrypsinogen
- Chromatography, Gel
- Cattle
- Animals
- Analytical Chemistry
- 40 Engineering
Citation
APA
Chicago
ICMJE
MLA
NLM
Mitchell, N. S., Hagel, L., & Fernandez, E. J. (1997). In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A, 779(1–2), 73–89. https://doi.org/10.1016/s0021-9673(97)00457-3
Mitchell, N. S., L. Hagel, and E. J. Fernandez. “In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.” J Chromatogr A 779, no. 1–2 (August 29, 1997): 73–89. https://doi.org/10.1016/s0021-9673(97)00457-3.
Mitchell NS, Hagel L, Fernandez EJ. In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A. 1997 Aug 29;779(1–2):73–89.
Mitchell, N. S., et al. “In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging.” J Chromatogr A, vol. 779, no. 1–2, Aug. 1997, pp. 73–89. Pubmed, doi:10.1016/s0021-9673(97)00457-3.
Mitchell NS, Hagel L, Fernandez EJ. In situ analysis of protein chromatography and column efficiency using magnetic resonance imaging. J Chromatogr A. 1997 Aug 29;779(1–2):73–89.
Published In
J Chromatogr A
DOI
ISSN
0021-9673
Publication Date
August 29, 1997
Volume
779
Issue
1-2
Start / End Page
73 / 89
Location
Netherlands
Related Subject Headings
- Serum Albumin
- Osmolar Concentration
- Magnetic Resonance Imaging
- Gadolinium DTPA
- Chymotrypsinogen
- Chromatography, Gel
- Cattle
- Animals
- Analytical Chemistry
- 40 Engineering