Current technology for the derivation of pluripotent stem cell lines from human embryos.
Publication
, Journal Article
Hasegawa, K; Pomeroy, JE; Pera, MF
Published in: Cell Stem Cell
June 4, 2010
Technology for the derivation, propagation, and characterization of pluripotent stem cell lines from the human embryo has undergone considerable refinement and improvement since the first published description of human embryonic stem cells in 1998. In particular, there has been extensive effort to optimize protocols and develop defined culture systems with a view toward future clinical applications of embryonic stem cell-derived products. Here, we review the current status of methodology for human embryonic stem cell derivation and culture, and we highlight the challenges that remain for workers in the field.
Duke Scholars
Published In
Cell Stem Cell
DOI
EISSN
1875-9777
Publication Date
June 4, 2010
Volume
6
Issue
6
Start / End Page
521 / 531
Location
United States
Related Subject Headings
- Pluripotent Stem Cells
- Humans
- Extracellular Matrix
- Embryo, Mammalian
- Developmental Biology
- Costs and Cost Analysis
- Cell Line
- Cell Culture Techniques
- Biotechnology
- 32 Biomedical and clinical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Hasegawa, K., Pomeroy, J. E., & Pera, M. F. (2010). Current technology for the derivation of pluripotent stem cell lines from human embryos. Cell Stem Cell, 6(6), 521–531. https://doi.org/10.1016/j.stem.2010.05.010
Hasegawa, Kouichi, Jordan E. Pomeroy, and Martin F. Pera. “Current technology for the derivation of pluripotent stem cell lines from human embryos.” Cell Stem Cell 6, no. 6 (June 4, 2010): 521–31. https://doi.org/10.1016/j.stem.2010.05.010.
Hasegawa K, Pomeroy JE, Pera MF. Current technology for the derivation of pluripotent stem cell lines from human embryos. Cell Stem Cell. 2010 Jun 4;6(6):521–31.
Hasegawa, Kouichi, et al. “Current technology for the derivation of pluripotent stem cell lines from human embryos.” Cell Stem Cell, vol. 6, no. 6, June 2010, pp. 521–31. Pubmed, doi:10.1016/j.stem.2010.05.010.
Hasegawa K, Pomeroy JE, Pera MF. Current technology for the derivation of pluripotent stem cell lines from human embryos. Cell Stem Cell. 2010 Jun 4;6(6):521–531.
Published In
Cell Stem Cell
DOI
EISSN
1875-9777
Publication Date
June 4, 2010
Volume
6
Issue
6
Start / End Page
521 / 531
Location
United States
Related Subject Headings
- Pluripotent Stem Cells
- Humans
- Extracellular Matrix
- Embryo, Mammalian
- Developmental Biology
- Costs and Cost Analysis
- Cell Line
- Cell Culture Techniques
- Biotechnology
- 32 Biomedical and clinical sciences