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Microbial colonization is required for normal neurobehavioral development in zebrafish.

Publication ,  Journal Article
Phelps, D; Brinkman, NE; Keely, SP; Anneken, EM; Catron, TR; Betancourt, D; Wood, CE; Espenschied, ST; Rawls, JF; Tal, T
Published in: Sci Rep
September 11, 2017

Changes in resident microbiota may have wide-ranging effects on human health. We investigated whether early life microbial disruption alters neurodevelopment and behavior in larval zebrafish. Conventionally colonized, axenic, and axenic larvae colonized at 1 day post fertilization (dpf) were evaluated using a standard locomotor assay. At 10 dpf, axenic zebrafish exhibited hyperactivity compared to conventionalized and conventionally colonized controls. Impairment of host colonization using antibiotics also caused hyperactivity in conventionally colonized larvae. To determine whether there is a developmental requirement for microbial colonization, axenic embryos were serially colonized on 1, 3, 6, or 9 dpf and evaluated on 10 dpf. Normal activity levels were observed in axenic larvae colonized on 1-6 dpf, but not on 9 dpf. Colonization of axenic embryos at 1 dpf with individual bacterial species Aeromonas veronii or Vibrio cholerae was sufficient to block locomotor hyperactivity at 10 dpf. Exposure to heat-killed bacteria or microbe-associated molecular patterns pam3CSK4 or Poly(I:C) was not sufficient to block hyperactivity in axenic larvae. These data show that microbial colonization during early life is required for normal neurobehavioral development and support the concept that antibiotics and other environmental chemicals may exert neurobehavioral effects via disruption of host-associated microbial communities.

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

September 11, 2017

Volume

7

Issue

1

Start / End Page

11244

Location

England

Related Subject Headings

  • Zebrafish
  • Vibrio cholerae
  • Nervous System
  • Locomotion
  • Larva
  • Gastrointestinal Microbiome
  • Embryo, Nonmammalian
  • Behavior, Animal
  • Anti-Bacterial Agents
  • Animals
 

Citation

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Phelps, D., Brinkman, N. E., Keely, S. P., Anneken, E. M., Catron, T. R., Betancourt, D., … Tal, T. (2017). Microbial colonization is required for normal neurobehavioral development in zebrafish. Sci Rep, 7(1), 11244. https://doi.org/10.1038/s41598-017-10517-5
Phelps, Drake, Nichole E. Brinkman, Scott P. Keely, Emily M. Anneken, Tara R. Catron, Doris Betancourt, Charles E. Wood, Scott T. Espenschied, John F. Rawls, and Tamara Tal. “Microbial colonization is required for normal neurobehavioral development in zebrafish.Sci Rep 7, no. 1 (September 11, 2017): 11244. https://doi.org/10.1038/s41598-017-10517-5.
Phelps D, Brinkman NE, Keely SP, Anneken EM, Catron TR, Betancourt D, et al. Microbial colonization is required for normal neurobehavioral development in zebrafish. Sci Rep. 2017 Sep 11;7(1):11244.
Phelps, Drake, et al. “Microbial colonization is required for normal neurobehavioral development in zebrafish.Sci Rep, vol. 7, no. 1, Sept. 2017, p. 11244. Pubmed, doi:10.1038/s41598-017-10517-5.
Phelps D, Brinkman NE, Keely SP, Anneken EM, Catron TR, Betancourt D, Wood CE, Espenschied ST, Rawls JF, Tal T. Microbial colonization is required for normal neurobehavioral development in zebrafish. Sci Rep. 2017 Sep 11;7(1):11244.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

September 11, 2017

Volume

7

Issue

1

Start / End Page

11244

Location

England

Related Subject Headings

  • Zebrafish
  • Vibrio cholerae
  • Nervous System
  • Locomotion
  • Larva
  • Gastrointestinal Microbiome
  • Embryo, Nonmammalian
  • Behavior, Animal
  • Anti-Bacterial Agents
  • Animals