Lessons from single-cell transcriptome analysis of oxygen-sensing cells.
The advent of single-cell RNA-sequencing (RNA-Seq) technology has enabled transcriptome profiling of individual cells. Comprehensive gene expression analysis at the single-cell level has proven to be effective in characterizing the most fundamental aspects of cellular function and identity. This unbiased approach is revolutionary for small and/or heterogeneous tissues like oxygen-sensing cells in identifying key molecules. Here, we review the major methods of current single-cell RNA-Seq technology. We discuss how this technology has advanced the understanding of oxygen-sensing glomus cells in the carotid body and helped uncover novel oxygen-sensing cells and mechanisms in the mice olfactory system. We conclude by providing our perspective on future single-cell RNA-Seq research directed at oxygen-sensing cells.
Duke Scholars
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Related Subject Headings
- Single-Cell Analysis
- Oxygen
- Olfactory Receptor Neurons
- Neurology & Neurosurgery
- Humans
- Gene Expression Profiling
- Carotid Body
- Animals
- 3208 Medical physiology
- 1116 Medical Physiology
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Single-Cell Analysis
- Oxygen
- Olfactory Receptor Neurons
- Neurology & Neurosurgery
- Humans
- Gene Expression Profiling
- Carotid Body
- Animals
- 3208 Medical physiology
- 1116 Medical Physiology