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Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.

Publication ,  Journal Article
Hoffmann, R; Grabińska, K; Guan, Z; Sessa, WC; Neiman, AM
Published in: Genetics
December 2017

Dolichols are isoprenoid lipids of varying length that act as sugar carriers in glycosylation reactions in the endoplasmic reticulum. In Saccharomyces cerevisiae, there are two cis-prenyltransferases that synthesize polyprenol-an essential precursor to dolichol. These enzymes are heterodimers composed of Nus1 and either Rer2 or Srt1. Rer2-Nus1 and Srt1-Nus1 can both generate dolichol in vegetative cells, but srt1∆ cells grow normally while rer2∆ grows very slowly, indicating that Rer2-Nus1 is the primary enzyme used in mitotically dividing cells. In contrast, SRT1 performs an important function in sporulating cells, where the haploid genomes created by meiosis are packaged into spores. The spore wall is a multilaminar structure and SRT1 is required for the generation of the outer chitosan and dityrosine layers of the spore wall. Srt1 specifically localizes to lipid droplets associated with spore walls, and, during sporulation there is an SRT1-dependent increase in long-chain polyprenols and dolichols in these lipid droplets. Synthesis of chitin by Chs3, the chitin synthase responsible for chitosan layer formation, is dependent on the cis-prenyltransferase activity of Srt1, indicating that polyprenols are necessary to coordinate assembly of the spore wall layers. This work shows that a developmentally regulated cis-prenyltransferase can produce polyprenols that function in cellular processes besides protein glycosylation.

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Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

December 2017

Volume

207

Issue

4

Start / End Page

1371 / 1386

Location

United States

Related Subject Headings

  • Tretinoin
  • Spores, Fungal
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Meiosis
  • Haploidy
  • Endoplasmic Reticulum
  • Dolichols
  • Dimethylallyltranstransferase
  • Developmental Biology
 

Citation

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Hoffmann, R., Grabińska, K., Guan, Z., Sessa, W. C., & Neiman, A. M. (2017). Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae. Genetics, 207(4), 1371–1386. https://doi.org/10.1534/genetics.117.300322
Hoffmann, Reuben, Kariona Grabińska, Ziqiang Guan, William C. Sessa, and Aaron M. Neiman. “Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.Genetics 207, no. 4 (December 2017): 1371–86. https://doi.org/10.1534/genetics.117.300322.
Hoffmann R, Grabińska K, Guan Z, Sessa WC, Neiman AM. Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae. Genetics. 2017 Dec;207(4):1371–86.
Hoffmann, Reuben, et al. “Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.Genetics, vol. 207, no. 4, Dec. 2017, pp. 1371–86. Pubmed, doi:10.1534/genetics.117.300322.
Hoffmann R, Grabińska K, Guan Z, Sessa WC, Neiman AM. Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae. Genetics. 2017 Dec;207(4):1371–1386.

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

December 2017

Volume

207

Issue

4

Start / End Page

1371 / 1386

Location

United States

Related Subject Headings

  • Tretinoin
  • Spores, Fungal
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Meiosis
  • Haploidy
  • Endoplasmic Reticulum
  • Dolichols
  • Dimethylallyltranstransferase
  • Developmental Biology