Droplet Size-Aware and Error-Correcting Sample Preparation Using Micro-Electrode-Dot-Array Digital Microfluidic Biochips.
Sample preparation in digital microfluidics refers to the generation of droplets with target concentrations for on-chip biochemical applications. In recent years, digital microfluidic biochips (DMFBs) have been adopted as a platform for sample preparation. However, there remain two major problems associated with sample preparation on a conventional DMFB. First, only a (1:1) mixing/splitting model can be used, leading to an increase in the number of fluidic operations required for sample preparation. Second, only a limited number of sensors can be integrated on a conventional DMFB; as a result, the latency for error detection during sample preparation is significant. To overcome these drawbacks, we adopt a next generation DMFB platform, referred to as micro-electrode-dot-array (MEDA), for sample preparation. We propose the first sample-preparation method that exploits the MEDA-specific advantages of fine-grained control of droplet sizes and real-time droplet sensing. Experimental demonstration using a fabricated MEDA biochip and simulation results highlight the effectiveness of the proposed sample-preparation method.
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Related Subject Headings
- Microfluidics
- Microfluidic Analytical Techniques
- Microarray Analysis
- Electrodes
- Electrical & Electronic Engineering
- 4009 Electronics, sensors and digital hardware
- 4003 Biomedical engineering
- 0906 Electrical and Electronic Engineering
- 0903 Biomedical Engineering
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Microfluidics
- Microfluidic Analytical Techniques
- Microarray Analysis
- Electrodes
- Electrical & Electronic Engineering
- 4009 Electronics, sensors and digital hardware
- 4003 Biomedical engineering
- 0906 Electrical and Electronic Engineering
- 0903 Biomedical Engineering