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Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies.

Publication ,  Journal Article
Ramesh, A; Darko, S; Hua, A; Overman, G; Ransier, A; Francica, JR; Trama, A; Tomaras, GD; Haynes, BF; Douek, DC; Kepler, TB
Published in: Front Immunol
2017

The rhesus macaque is a critically important animal model in biomedical research, most recently playing a key role in the development of vaccines against human immunodeficiency virus-1. Nevertheless, the immunoglobulin (Ig) loci of macaques are as yet incompletely determined and our understanding of differences between human and macaque humoral immunity remains deficient. We completed a high-coverage, high-quality whole genome sequencing and assembly project with a single rhesus macaque of Indian origin, and partial genome assemblies using genomic molecular targeting of the Ig loci in nine other rhesus macaques of Indian origin. These data indicate that the macaque Ig loci are substantially more diverse than those in humans, including greater sequence diversity and copy-number variation between individuals. It appears likely that such copy-number variation even occurs between allelic loci within individuals. Different Ig gene families in the macaque show distinct relationships to the corresponding human gene families and appear to evolve under different mechanisms. These results raise intriguing questions about the evolution of antigen receptors in primates but also have important practical implications for the design and interpretation of biomedical studies.

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Published In

Front Immunol

DOI

ISSN

1664-3224

Publication Date

2017

Volume

8

Start / End Page

1407

Location

Switzerland

Related Subject Headings

  • 3204 Immunology
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 1108 Medical Microbiology
  • 1107 Immunology
 

Citation

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Ramesh, A., Darko, S., Hua, A., Overman, G., Ransier, A., Francica, J. R., … Kepler, T. B. (2017). Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies. Front Immunol, 8, 1407. https://doi.org/10.3389/fimmu.2017.01407
Ramesh, Akshaya, Sam Darko, Axin Hua, Glenn Overman, Amy Ransier, Joseph R. Francica, Ashley Trama, et al. “Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies.Front Immunol 8 (2017): 1407. https://doi.org/10.3389/fimmu.2017.01407.
Ramesh A, Darko S, Hua A, Overman G, Ransier A, Francica JR, et al. Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies. Front Immunol. 2017;8:1407.
Ramesh, Akshaya, et al. “Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies.Front Immunol, vol. 8, 2017, p. 1407. Pubmed, doi:10.3389/fimmu.2017.01407.
Ramesh A, Darko S, Hua A, Overman G, Ransier A, Francica JR, Trama A, Tomaras GD, Haynes BF, Douek DC, Kepler TB. Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies. Front Immunol. 2017;8:1407.

Published In

Front Immunol

DOI

ISSN

1664-3224

Publication Date

2017

Volume

8

Start / End Page

1407

Location

Switzerland

Related Subject Headings

  • 3204 Immunology
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 1108 Medical Microbiology
  • 1107 Immunology