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Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer.

Publication ,  Journal Article
Zhang, L; Shi, J; Feng, J; Klocker, H; Lee, C; Zhang, J
Published in: Prostate cancer and prostatic diseases
January 2004

The type IV collagenases/gelatinases matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) play an important role in cancer invasion and metastasis. In the present study, we measured the expression of mRNAs and enzymatic activities of MMP-9 and -2 in prostate tissues and serum samples from men with or without prostate cancer.A total of 44 tissue samples (three from healthy volunteers, 21 from patients with benign prostate hyperplasia, 10 from patients with localized prostate cancer and 10 from patients with metastatic disease) and 71 serum samples were collected (20 from healthy volunteers, 26 from patients with benign prostatic hyperplasia, 10 from patients with localized cancer, 15 from patients with metastatic cancer). The level of mRNA for MMP-2 and -9 was determined by semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). The enzymatic activity of MMPs was determined by zymography.Expression of MMP-9 mRNA was significantly higher in malignant than in nonmalignant prostate tissues (P < 0.001), while no significant difference of MMP-2 expression was detected in different prostate tissues. Results of zymography showed that there was significant difference in the enzymatic activity of MMP-9, but not MMP-2, among normal prostate, BPH, localized and metastatic prostate cancer tissues, serum samples (P < 0.05). The active form of MMP-2, with a molecular mass of 62 kDa, was detected in normal prostate, BPH and prostate cancer tissues, but not in the serum samples. Moreover, there was a significant difference in the ratio of the active form (62 kDa) and proform (72 kDa) of MMP-2 among normal, BPH and prostate cancer tissues. This ratio was further increased in metastatic prostate cancer tissues.The activity of MMP-9 and the ratio of active form/proform of MMP-2 are associated with the progression and metastasis of prostate cancer.

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Published In

Prostate cancer and prostatic diseases

DOI

EISSN

1476-5608

ISSN

1365-7852

Publication Date

January 2004

Volume

7

Issue

4

Start / End Page

327 / 332

Related Subject Headings

  • Urology & Nephrology
  • RNA, Messenger
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Prostate
  • Neoplasm Staging
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2
 

Citation

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Zhang, L., Shi, J., Feng, J., Klocker, H., Lee, C., & Zhang, J. (2004). Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer. Prostate Cancer and Prostatic Diseases, 7(4), 327–332. https://doi.org/10.1038/sj.pcan.4500750
Zhang, L., J. Shi, J. Feng, H. Klocker, C. Lee, and J. Zhang. “Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer.Prostate Cancer and Prostatic Diseases 7, no. 4 (January 2004): 327–32. https://doi.org/10.1038/sj.pcan.4500750.
Zhang L, Shi J, Feng J, Klocker H, Lee C, Zhang J. Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer. Prostate cancer and prostatic diseases. 2004 Jan;7(4):327–32.
Zhang, L., et al. “Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer.Prostate Cancer and Prostatic Diseases, vol. 7, no. 4, Jan. 2004, pp. 327–32. Epmc, doi:10.1038/sj.pcan.4500750.
Zhang L, Shi J, Feng J, Klocker H, Lee C, Zhang J. Type IV collagenase (matrix metalloproteinase-2 and -9) in prostate cancer. Prostate cancer and prostatic diseases. 2004 Jan;7(4):327–332.

Published In

Prostate cancer and prostatic diseases

DOI

EISSN

1476-5608

ISSN

1365-7852

Publication Date

January 2004

Volume

7

Issue

4

Start / End Page

327 / 332

Related Subject Headings

  • Urology & Nephrology
  • RNA, Messenger
  • Prostatic Neoplasms
  • Prostatic Hyperplasia
  • Prostate
  • Neoplasm Staging
  • Neoplasm Recurrence, Local
  • Middle Aged
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2