Skip to main content

Whole-genome fingerprint of the DNA methylome during human B cell differentiation.

Publication ,  Journal Article
Kulis, M; Merkel, A; Heath, S; Queirós, AC; Schuyler, RP; Castellano, G; Beekman, R; Raineri, E; Esteve, A; Clot, G; Verdaguer-Dot, N; Rico, D ...
Published in: Nat Genet
July 2015

We analyzed the DNA methylome of ten subpopulations spanning the entire B cell differentiation program by whole-genome bisulfite sequencing and high-density microarrays. We observed that non-CpG methylation disappeared upon B cell commitment, whereas CpG methylation changed extensively during B cell maturation, showing an accumulative pattern and affecting around 30% of all measured CpG sites. Early differentiation stages mainly displayed enhancer demethylation, which was associated with upregulation of key B cell transcription factors and affected multiple genes involved in B cell biology. Late differentiation stages, in contrast, showed extensive demethylation of heterochromatin and methylation gain at Polycomb-repressed areas, and genes with apparent functional impact in B cells were not affected. This signature, which has previously been linked to aging and cancer, was particularly widespread in mature cells with an extended lifespan. Comparing B cell neoplasms with their normal counterparts, we determined that they frequently acquire methylation changes in regions already undergoing dynamic methylation during normal B cell differentiation.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Genet

DOI

EISSN

1546-1718

Publication Date

July 2015

Volume

47

Issue

7

Start / End Page

746 / 756

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Leukemia, B-Cell
  • Humans
  • Genome, Human
  • Gene Expression Regulation, Leukemic
  • Epigenesis, Genetic
  • Developmental Biology
  • DNA Methylation
  • CpG Islands
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kulis, M., Merkel, A., Heath, S., Queirós, A. C., Schuyler, R. P., Castellano, G., … Martín-Subero, J. I. (2015). Whole-genome fingerprint of the DNA methylome during human B cell differentiation. Nat Genet, 47(7), 746–756. https://doi.org/10.1038/ng.3291
Kulis, Marta, Angelika Merkel, Simon Heath, Ana C. Queirós, Ronald P. Schuyler, Giancarlo Castellano, Renée Beekman, et al. “Whole-genome fingerprint of the DNA methylome during human B cell differentiation.Nat Genet 47, no. 7 (July 2015): 746–56. https://doi.org/10.1038/ng.3291.
Kulis M, Merkel A, Heath S, Queirós AC, Schuyler RP, Castellano G, et al. Whole-genome fingerprint of the DNA methylome during human B cell differentiation. Nat Genet. 2015 Jul;47(7):746–56.
Kulis, Marta, et al. “Whole-genome fingerprint of the DNA methylome during human B cell differentiation.Nat Genet, vol. 47, no. 7, July 2015, pp. 746–56. Pubmed, doi:10.1038/ng.3291.
Kulis M, Merkel A, Heath S, Queirós AC, Schuyler RP, Castellano G, Beekman R, Raineri E, Esteve A, Clot G, Verdaguer-Dot N, Duran-Ferrer M, Russiñol N, Vilarrasa-Blasi R, Ecker S, Pancaldi V, Rico D, Agueda L, Blanc J, Richardson D, Clarke L, Datta A, Pascual M, Agirre X, Prosper F, Alignani D, Paiva B, Caron G, Fest T, Muench MO, Fomin ME, Lee S-T, Wiemels JL, Valencia A, Gut M, Flicek P, Stunnenberg HG, Siebert R, Küppers R, Gut IG, Campo E, Martín-Subero JI. Whole-genome fingerprint of the DNA methylome during human B cell differentiation. Nat Genet. 2015 Jul;47(7):746–756.

Published In

Nat Genet

DOI

EISSN

1546-1718

Publication Date

July 2015

Volume

47

Issue

7

Start / End Page

746 / 756

Location

United States

Related Subject Headings

  • Sequence Analysis, DNA
  • Leukemia, B-Cell
  • Humans
  • Genome, Human
  • Gene Expression Regulation, Leukemic
  • Epigenesis, Genetic
  • Developmental Biology
  • DNA Methylation
  • CpG Islands
  • Cells, Cultured