Decoy mRNAs reduce beta-amyloid precursor protein mRNA in neuronal cells.
Overproduction of amyloid precursor protein (APP) and beta-amyloid likely contribute to neurodegeneration in Alzheimer's disease (AD). In an effort to understand neuronal APP gene regulation, we identified a 52 base element (52sce) immediately downstream from the stop codon that stabilizes APP mRNA. Deletion of this domain drastically destabilized APP mRNAs and reduced APP synthesis in vitro. Chimeric globin-APP mRNAs containing the globin coding sequence fused to the entire APP 3'-UTR, showed regulation similar to full-length APP mRNA. A variety of cytoplasmic lysates contain 52sce RNA binding activity, suggesting cis-trans interactions regulate the element's functionality. Finally, the overexpression of chimeric mRNAs, containing the GFP coding sequence and APP 3'-UTR, dramatically reduced endogenous APP steady-state levels in SH-SY5Y neuroblastoma cells and suggests a novel approach to reduce the amyloid burden in AD patients.
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Related Subject Headings
- Transfection
- Transcription, Genetic
- Time Factors
- Reverse Transcriptase Polymerase Chain Reaction
- RNA, Messenger
- Protein Biosynthesis
- Neurons
- Neurology & Neurosurgery
- Mutagenesis
- Models, Molecular
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Transfection
- Transcription, Genetic
- Time Factors
- Reverse Transcriptase Polymerase Chain Reaction
- RNA, Messenger
- Protein Biosynthesis
- Neurons
- Neurology & Neurosurgery
- Mutagenesis
- Models, Molecular