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High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A.

Publication ,  Journal Article
Pless, DD; Torres, ER; Reinke, EK; Bavari, S
Published in: Infection and immunity
January 2001

Monoclonal antibodies (MAbs) were prepared against the putative binding domain of botulinum neurotoxin A (BoNT/A), a nontoxic 50-kDa fragment. Initially, all fusion products were screened against the holotoxin BoNT/A and against the binding fragment, BoNT/A H(C). Eleven neutralizing hybridomas were cloned, and their specific binding to BoNT/A H(C) was demonstrated by surface plasmon resonance, with dissociation constants ranging from 0.9 to <0.06 nM. Epitope mapping by real-time surface plasmon resonance showed that the antibodies bound to at least two distinct regions of the BoNT/A H(C) fragment. These MAbs will be useful tools for studying BoNT/A interactions with its receptor, and they have potential diagnostic and therapeutic applications.

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Published In

Infection and immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

January 2001

Volume

69

Issue

1

Start / End Page

570 / 574

Related Subject Headings

  • Vaccination
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Epitope Mapping
  • Enzyme-Linked Immunosorbent Assay
  • Botulinum Toxins
  • Biosensing Techniques
  • Binding Sites
  • Antibody Affinity
 

Citation

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Pless, D. D., Torres, E. R., Reinke, E. K., & Bavari, S. (2001). High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. Infection and Immunity, 69(1), 570–574. https://doi.org/10.1128/iai.69.1.570-574.2001
Pless, D. D., E. R. Torres, E. K. Reinke, and S. Bavari. “High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A.Infection and Immunity 69, no. 1 (January 2001): 570–74. https://doi.org/10.1128/iai.69.1.570-574.2001.
Pless DD, Torres ER, Reinke EK, Bavari S. High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. Infection and immunity. 2001 Jan;69(1):570–4.
Pless, D. D., et al. “High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A.Infection and Immunity, vol. 69, no. 1, Jan. 2001, pp. 570–74. Epmc, doi:10.1128/iai.69.1.570-574.2001.
Pless DD, Torres ER, Reinke EK, Bavari S. High-affinity, protective antibodies to the binding domain of botulinum neurotoxin type A. Infection and immunity. 2001 Jan;69(1):570–574.

Published In

Infection and immunity

DOI

EISSN

1098-5522

ISSN

0019-9567

Publication Date

January 2001

Volume

69

Issue

1

Start / End Page

570 / 574

Related Subject Headings

  • Vaccination
  • Microbiology
  • Mice, Inbred BALB C
  • Mice
  • Epitope Mapping
  • Enzyme-Linked Immunosorbent Assay
  • Botulinum Toxins
  • Biosensing Techniques
  • Binding Sites
  • Antibody Affinity