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Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.

Publication ,  Journal Article
Zozulya, AL; Reinke, E; Baiu, DC; Karman, J; Sandor, M; Fabry, Z
Published in: Journal of immunology (Baltimore, Md. : 1950)
January 2007

Dendritic cells (DCs) accumulate in the CNS during inflammatory diseases, but the exact mechanism regulating their traffic into the CNS remains to be defined. We now report that MIP-1alpha increases the transmigration of bone marrow-derived, GFP-labeled DCs across brain microvessel endothelial cell monolayers. Furthermore, occludin, an important element of endothelial tight junctions, is reorganized when DCs migrate across brain capillary endothelial cell monolayers without causing significant changes in the barrier integrity as measured by transendothelial electrical resistance. We show that DCs produce matrix metalloproteinases (MMP) -2 and -9 and GM6001, an MMP inhibitor, decreases both baseline and MIP-1alpha-induced DC transmigration. These observations suggest that DC transmigration across brain endothelial cell monolayers is partly MMP dependent. The migrated DCs express higher levels of CD40, CD80, and CD86 costimulatory molecules and induce T cell proliferation, indicating that the transmigration of DCs across brain endothelial cell monolayers contributes to the maintenance of DC Ag-presenting function. The MMP dependence of DC migration across brain endothelial cell monolayers raises the possibility that MMP blockers may decrease the initiation of T cell recruitment and neuroinflammation in the CNS.

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Published In

Journal of immunology (Baltimore, Md. : 1950)

DOI

EISSN

1550-6606

ISSN

0022-1767

Publication Date

January 2007

Volume

178

Issue

1

Start / End Page

520 / 529

Related Subject Headings

  • Tight Junctions
  • Occludin
  • Mice, Transgenic
  • Mice
  • Membrane Proteins
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase Inhibitors
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2
  • Macrophage Inflammatory Proteins
 

Citation

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Zozulya, A. L., Reinke, E., Baiu, D. C., Karman, J., Sandor, M., & Fabry, Z. (2007). Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases. Journal of Immunology (Baltimore, Md. : 1950), 178(1), 520–529. https://doi.org/10.4049/jimmunol.178.1.520
Zozulya, Alla L., Emily Reinke, Dana C. Baiu, Jozsef Karman, Matyas Sandor, and Zsuzsanna Fabry. “Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.Journal of Immunology (Baltimore, Md. : 1950) 178, no. 1 (January 2007): 520–29. https://doi.org/10.4049/jimmunol.178.1.520.
Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z. Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases. Journal of immunology (Baltimore, Md : 1950). 2007 Jan;178(1):520–9.
Zozulya, Alla L., et al. “Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.Journal of Immunology (Baltimore, Md. : 1950), vol. 178, no. 1, Jan. 2007, pp. 520–29. Epmc, doi:10.4049/jimmunol.178.1.520.
Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z. Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases. Journal of immunology (Baltimore, Md : 1950). 2007 Jan;178(1):520–529.

Published In

Journal of immunology (Baltimore, Md. : 1950)

DOI

EISSN

1550-6606

ISSN

0022-1767

Publication Date

January 2007

Volume

178

Issue

1

Start / End Page

520 / 529

Related Subject Headings

  • Tight Junctions
  • Occludin
  • Mice, Transgenic
  • Mice
  • Membrane Proteins
  • Matrix Metalloproteinases
  • Matrix Metalloproteinase Inhibitors
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase 2
  • Macrophage Inflammatory Proteins