Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.
Publication
, Journal Article
Woroniecka, K; Chongsathidkiet, P; Elsamadicy, A; Farber, H; Cui, X; Fecci, PE
Published in: Methods Mol Biol
2018
We describe an isolation method of tumor-infiltrating lymphocytes (TILs) from glioblastoma tumors for the purpose of analysis by flow cytometry. This protocol is unique from many others in that the use of a selective lymphocyte isolation procedure, such as a Ficoll or Percoll gradient, is not used. We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isolation procedures can significantly decrease lymphocyte yield from already rare populations.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1741
Start / End Page
221 / 226
Location
United States
Related Subject Headings
- Mice
- Lymphocytes, Tumor-Infiltrating
- Immunophenotyping
- Immunohistochemistry
- Humans
- Glioblastoma
- Flow Cytometry
- Developmental Biology
- Cytokines
- Brain Neoplasms
Citation
APA
Chicago
ICMJE
MLA
NLM
Woroniecka, K., Chongsathidkiet, P., Elsamadicy, A., Farber, H., Cui, X., & Fecci, P. E. (2018). Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol, 1741, 221–226. https://doi.org/10.1007/978-1-4939-7659-1_18
Woroniecka, Karolina, Pakawat Chongsathidkiet, Aladine Elsamadicy, Harrison Farber, Xiuyu Cui, and Peter E. Fecci. “Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.” Methods Mol Biol 1741 (2018): 221–26. https://doi.org/10.1007/978-1-4939-7659-1_18.
Woroniecka K, Chongsathidkiet P, Elsamadicy A, Farber H, Cui X, Fecci PE. Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol. 2018;1741:221–6.
Woroniecka, Karolina, et al. “Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.” Methods Mol Biol, vol. 1741, 2018, pp. 221–26. Pubmed, doi:10.1007/978-1-4939-7659-1_18.
Woroniecka K, Chongsathidkiet P, Elsamadicy A, Farber H, Cui X, Fecci PE. Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol. 2018;1741:221–226.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1741
Start / End Page
221 / 226
Location
United States
Related Subject Headings
- Mice
- Lymphocytes, Tumor-Infiltrating
- Immunophenotyping
- Immunohistochemistry
- Humans
- Glioblastoma
- Flow Cytometry
- Developmental Biology
- Cytokines
- Brain Neoplasms