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Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.

Publication ,  Journal Article
Woroniecka, K; Chongsathidkiet, P; Elsamadicy, A; Farber, H; Cui, X; Fecci, PE
Published in: Methods Mol Biol
2018

We describe an isolation method of tumor-infiltrating lymphocytes (TILs) from glioblastoma tumors for the purpose of analysis by flow cytometry. This protocol is unique from many others in that the use of a selective lymphocyte isolation procedure, such as a Ficoll or Percoll gradient, is not used. We find that staining of TILs and analysis by flow cytometry is not affected by the presence of heterogeneous populations, while other selective isolation procedures can significantly decrease lymphocyte yield from already rare populations.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1741

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Mice
  • Lymphocytes, Tumor-Infiltrating
  • Immunophenotyping
  • Immunohistochemistry
  • Humans
  • Glioblastoma
  • Flow Cytometry
  • Developmental Biology
  • Cytokines
  • Brain Neoplasms
 

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MLA
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Woroniecka, K., Chongsathidkiet, P., Elsamadicy, A., Farber, H., Cui, X., & Fecci, P. E. (2018). Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol, 1741, 221–226. https://doi.org/10.1007/978-1-4939-7659-1_18
Woroniecka, Karolina, Pakawat Chongsathidkiet, Aladine Elsamadicy, Harrison Farber, Xiuyu Cui, and Peter E. Fecci. “Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.Methods Mol Biol 1741 (2018): 221–26. https://doi.org/10.1007/978-1-4939-7659-1_18.
Woroniecka K, Chongsathidkiet P, Elsamadicy A, Farber H, Cui X, Fecci PE. Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol. 2018;1741:221–6.
Woroniecka, Karolina, et al. “Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma.Methods Mol Biol, vol. 1741, 2018, pp. 221–26. Pubmed, doi:10.1007/978-1-4939-7659-1_18.
Woroniecka K, Chongsathidkiet P, Elsamadicy A, Farber H, Cui X, Fecci PE. Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma. Methods Mol Biol. 2018;1741:221–226.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1741

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Mice
  • Lymphocytes, Tumor-Infiltrating
  • Immunophenotyping
  • Immunohistochemistry
  • Humans
  • Glioblastoma
  • Flow Cytometry
  • Developmental Biology
  • Cytokines
  • Brain Neoplasms