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Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry.

Publication ,  Journal Article
Kim, T; London, A; Kharasch, ED
Published in: J Pharm Biomed Anal
June 1, 2011

A fast, sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of alfentanil and midazolam in human plasma has been developed and validated. Alfentanil and midazolam were extracted from plasma using a mixed-mode cation exchange solid phase extraction method, with recoveries of both compounds greater than 80% at 3 different concentrations (1, 10 and 100ng/ml). Compounds were analyzed on a C(18) column with a water and methanol mobile phase gradient with acetic acid as an additive, at a flow rate of 0.3ml/min. The working assay range was linear from 0.25 to 100ng/ml for each compound. The signal to noise ratio was 80 and 40 for alfentanil and midazolam, respectively, at the lowest concentration calibration standard, with less than 10% matrix suppression by human plasma at this concentration. Alfentanil and midazolam were stable in human plasma during storage at -80°C, processing, and analysis. The procedure was validated and applied to the analysis of plasma samples from healthy human subjects administered oral and intravenous alfentanil and midazolam.

Duke Scholars

Published In

J Pharm Biomed Anal

DOI

EISSN

1873-264X

Publication Date

June 1, 2011

Volume

55

Issue

3

Start / End Page

487 / 493

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Specimen Handling
  • Solid Phase Extraction
  • Reproducibility of Results
  • Reference Standards
  • Midazolam
  • Injections, Intravenous
  • Humans
  • Drug Storage
  • Drug Stability
 

Citation

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Kim, T., London, A., & Kharasch, E. D. (2011). Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry. J Pharm Biomed Anal, 55(3), 487–493. https://doi.org/10.1016/j.jpba.2011.01.040
Kim, Thomas, Amy London, and Evan D. Kharasch. “Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry.J Pharm Biomed Anal 55, no. 3 (June 1, 2011): 487–93. https://doi.org/10.1016/j.jpba.2011.01.040.
Kim, Thomas, et al. “Simultaneous determination of alfentanil and midazolam in human plasma using liquid chromatography and tandem mass spectrometry.J Pharm Biomed Anal, vol. 55, no. 3, June 2011, pp. 487–93. Pubmed, doi:10.1016/j.jpba.2011.01.040.
Journal cover image

Published In

J Pharm Biomed Anal

DOI

EISSN

1873-264X

Publication Date

June 1, 2011

Volume

55

Issue

3

Start / End Page

487 / 493

Location

England

Related Subject Headings

  • Tandem Mass Spectrometry
  • Specimen Handling
  • Solid Phase Extraction
  • Reproducibility of Results
  • Reference Standards
  • Midazolam
  • Injections, Intravenous
  • Humans
  • Drug Storage
  • Drug Stability