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Long-term optical brain imaging in live adult fruit flies.

Publication ,  Journal Article
Huang, C; Maxey, JR; Sinha, S; Savall, J; Gong, Y; Schnitzer, MJ
Published in: Nature communications
February 2018

Time-lapse in vivo microscopy studies of cellular morphology and physiology are crucial toward understanding brain function but have been infeasible in the fruit fly, a key model species. Here we use laser microsurgery to create a chronic fly preparation for repeated imaging of neural architecture and dynamics for up to 50 days. In fly mushroom body neurons, we track axonal boutons for 10 days and record odor-evoked calcium transients over 7 weeks. Further, by using voltage imaging to resolve individual action potentials, we monitor spiking plasticity in dopamine neurons of flies undergoing mechanical stress. After 24 h of stress, PPL1-α'3 but not PPL1-α'2α2 dopamine neurons have elevated spike rates. Overall, our chronic preparation is compatible with a broad range of optical techniques and enables longitudinal studies of many biological questions that could not be addressed before in live flies.

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Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

February 2018

Volume

9

Issue

1

Start / End Page

872

Related Subject Headings

  • Time-Lapse Imaging
  • Stress, Mechanical
  • Neuroimaging
  • Mushroom Bodies
  • Microsurgery
  • Microscopy
  • Male
  • Female
  • Drosophila melanogaster
  • Dopaminergic Neurons
 

Citation

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Huang, C., Maxey, J. R., Sinha, S., Savall, J., Gong, Y., & Schnitzer, M. J. (2018). Long-term optical brain imaging in live adult fruit flies. Nature Communications, 9(1), 872. https://doi.org/10.1038/s41467-018-02873-1
Huang, Cheng, Jessica R. Maxey, Supriyo Sinha, Joan Savall, Yiyang Gong, and Mark J. Schnitzer. “Long-term optical brain imaging in live adult fruit flies.Nature Communications 9, no. 1 (February 2018): 872. https://doi.org/10.1038/s41467-018-02873-1.
Huang C, Maxey JR, Sinha S, Savall J, Gong Y, Schnitzer MJ. Long-term optical brain imaging in live adult fruit flies. Nature communications. 2018 Feb;9(1):872.
Huang, Cheng, et al. “Long-term optical brain imaging in live adult fruit flies.Nature Communications, vol. 9, no. 1, Feb. 2018, p. 872. Epmc, doi:10.1038/s41467-018-02873-1.
Huang C, Maxey JR, Sinha S, Savall J, Gong Y, Schnitzer MJ. Long-term optical brain imaging in live adult fruit flies. Nature communications. 2018 Feb;9(1):872.

Published In

Nature communications

DOI

EISSN

2041-1723

ISSN

2041-1723

Publication Date

February 2018

Volume

9

Issue

1

Start / End Page

872

Related Subject Headings

  • Time-Lapse Imaging
  • Stress, Mechanical
  • Neuroimaging
  • Mushroom Bodies
  • Microsurgery
  • Microscopy
  • Male
  • Female
  • Drosophila melanogaster
  • Dopaminergic Neurons