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Phenotyping Tumor-Associated Macrophages.

Publication ,  Journal Article
Ian Cumming, R; Yu, Y-RA
Published in: Methods Mol Biol
2018

Multi-parametric flow cytometry of tumor-bearing murine nonlymphoid tissue allows for characterization, isolation, and examination of immune cell composition, phenotype, and function. Here we describe an approach to process nonlymphoid tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This panel can be used to phenotype tumor-associated macrophages.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1784

Start / End Page

99 / 109

Location

United States

Related Subject Headings

  • Phenotype
  • Monocytes
  • Mice
  • Macrophages
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Dendritic Cells
  • Animals
 

Citation

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Ian Cumming, R., & Yu, Y.-R. (2018). Phenotyping Tumor-Associated Macrophages. Methods Mol Biol, 1784, 99–109. https://doi.org/10.1007/978-1-4939-7837-3_10
Ian Cumming, R., and Yen-Rei A. Yu. “Phenotyping Tumor-Associated Macrophages.Methods Mol Biol 1784 (2018): 99–109. https://doi.org/10.1007/978-1-4939-7837-3_10.
Ian Cumming R, Yu Y-RA. Phenotyping Tumor-Associated Macrophages. Methods Mol Biol. 2018;1784:99–109.
Ian Cumming, R., and Yen-Rei A. Yu. “Phenotyping Tumor-Associated Macrophages.Methods Mol Biol, vol. 1784, 2018, pp. 99–109. Pubmed, doi:10.1007/978-1-4939-7837-3_10.
Ian Cumming R, Yu Y-RA. Phenotyping Tumor-Associated Macrophages. Methods Mol Biol. 2018;1784:99–109.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1784

Start / End Page

99 / 109

Location

United States

Related Subject Headings

  • Phenotype
  • Monocytes
  • Mice
  • Macrophages
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Dendritic Cells
  • Animals