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Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV.

Publication ,  Journal Article
Dong, X-H; Ho, M-H; Liu, B; Hildreth, J; Dash, C; Goodwin, JS; Balasubramaniam, M; Chen, C-H; Xie, H
Published in: Sci Rep
June 11, 2018

The association between mucosal microbiota and HIV-1 infection has garnered great attention in the field of HIV-1 research. Previously, we reported a receptor-independent HIV-1 entry into epithelial cells mediated by a Gram-negative invasive bacterium, Porphyromonas gingivalis. Here, we present evidence showing that P. gingivalis outer membrane vesicles (OMVs) promote mucosal transmission of HIV-1. We demonstrated, using the Dynabeads technology, a specific interaction between HIV-1 and P. gingivalis OMVs which led to an OMV-dependent viral entry into oral epithelial cells. HIV-1 was detected in human oral keratinocytes (HOKs) after a 20 minute exposure to the HIV-vesicle complexes. After entry, most of the complexes appeared to dissociate, HIV-1 was reverse-transcribed, and viral DNA was integrated into the genome of HOKs. Meanwhile, some of the complexes exited the original host and re-entered neighboring HOKs and permissive cells of HIV-1. Moreover, P. gingivalis vesicles enhanced HIV-1 infection of MT4 cells at low infecting doses that are not able to establish an efficient infection alone. These findings suggest that invasive bacteria and their OMVs with ability to interact with HIV-1 may serve as a vehicle to translocate HIV through the mucosa, establish mucosal transmission of HIV-1, and enhance HIV-1 infectivity.

Duke Scholars

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Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 11, 2018

Volume

8

Issue

1

Start / End Page

8812

Location

England

Related Subject Headings

  • Virus Internalization
  • Porphyromonas gingivalis
  • Models, Theoretical
  • Humans
  • HIV-1
  • HIV Infections
  • Extracellular Vesicles
  • Epithelial Cells
  • Disease Transmission, Infectious
  • Cells, Cultured
 

Citation

APA
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Dong, X.-H., Ho, M.-H., Liu, B., Hildreth, J., Dash, C., Goodwin, J. S., … Xie, H. (2018). Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV. Sci Rep, 8(1), 8812. https://doi.org/10.1038/s41598-018-27284-6
Dong, Xin-Hong, Meng-Hsuan Ho, Bindong Liu, James Hildreth, Chandravanu Dash, J Shawn Goodwin, Muthukumar Balasubramaniam, Chin-Ho Chen, and Hua Xie. “Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV.Sci Rep 8, no. 1 (June 11, 2018): 8812. https://doi.org/10.1038/s41598-018-27284-6.
Dong X-H, Ho M-H, Liu B, Hildreth J, Dash C, Goodwin JS, et al. Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV. Sci Rep. 2018 Jun 11;8(1):8812.
Dong, Xin-Hong, et al. “Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV.Sci Rep, vol. 8, no. 1, June 2018, p. 8812. Pubmed, doi:10.1038/s41598-018-27284-6.
Dong X-H, Ho M-H, Liu B, Hildreth J, Dash C, Goodwin JS, Balasubramaniam M, Chen C-H, Xie H. Role of Porphyromonas gingivalis outer membrane vesicles in oral mucosal transmission of HIV. Sci Rep. 2018 Jun 11;8(1):8812.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

June 11, 2018

Volume

8

Issue

1

Start / End Page

8812

Location

England

Related Subject Headings

  • Virus Internalization
  • Porphyromonas gingivalis
  • Models, Theoretical
  • Humans
  • HIV-1
  • HIV Infections
  • Extracellular Vesicles
  • Epithelial Cells
  • Disease Transmission, Infectious
  • Cells, Cultured