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High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control.

Publication ,  Journal Article
Akulian, JA; Pipeling, MR; John, ER; Orens, JB; Lechtzin, N; McDyer, JF
Published in: Am J Transplant
January 2013

The maintenance of CMV-specific T cell memory in lung transplant recipients (LTRs) is critical for host defense and allograft durability, particularly in donor(+) /recipient(-) (D(+) R(-) ) individuals who demonstrate increased mortality. We studied CD4(+) and CD8(+) CMV-specific memory responses to phosphoprotein 65 (pp65) in a prospective cohort of 18 D(+) R(-) LTRs, from bronchoalveolar lavage (BAL)-obtained lung mononuclear cells (LMNC) and PBMC. Unexpectedly, pp65-specific CD4(+) and CD8(+) IFN-γ memory responses from LMNC were similar, in contrast to persistent CD8(+) predominance in PBMC. Unlike the pulmonary CD8(+) predominance during acute primary infection, compartmental equalization occurred in the CMV-specific CD8(+) memory pool during chronic infection, whereas CMV-specific CD4(+) memory was enriched in the bronchoalveolar space. Moreover, CMV-specific CD4(+) memory T cells with multifunctional production of IFN-γ, TNF-α, IL-2 and MIP-1β were significantly increased in LMNCs, in contrast to similar intercompartmental CD8(+) memory function. Moreover, the absolute number of CMV-specific CD4(+) IFN-γ(+) memory cells in BAL was significantly increased in LTRs exhibiting viral control compared to those with CMV early antigen positivity. Collectively, these data demonstrate both preferential distribution and functional quality of CMV-specific CD4(+) memory in the lung allograft during chronic infection, and show an important association with CMV mucosal immunity and viral control.

Duke Scholars

Published In

Am J Transplant

DOI

EISSN

1600-6143

Publication Date

January 2013

Volume

13

Issue

1

Start / End Page

146 / 156

Location

United States

Related Subject Headings

  • Surgery
  • Prospective Studies
  • Middle Aged
  • Male
  • Lung Transplantation
  • Interferon-gamma
  • Immunologic Memory
  • Immunity, Mucosal
  • Humans
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Akulian, J. A., Pipeling, M. R., John, E. R., Orens, J. B., Lechtzin, N., & McDyer, J. F. (2013). High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control. Am J Transplant, 13(1), 146–156. https://doi.org/10.1111/j.1600-6143.2012.04282.x
Akulian, J. A., M. R. Pipeling, E. R. John, J. B. Orens, N. Lechtzin, and J. F. McDyer. “High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control.Am J Transplant 13, no. 1 (January 2013): 146–56. https://doi.org/10.1111/j.1600-6143.2012.04282.x.
Akulian JA, Pipeling MR, John ER, Orens JB, Lechtzin N, McDyer JF. High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control. Am J Transplant. 2013 Jan;13(1):146–56.
Akulian, J. A., et al. “High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control.Am J Transplant, vol. 13, no. 1, Jan. 2013, pp. 146–56. Pubmed, doi:10.1111/j.1600-6143.2012.04282.x.
Akulian JA, Pipeling MR, John ER, Orens JB, Lechtzin N, McDyer JF. High-quality CMV-specific CD4+ memory is enriched in the lung allograft and is associated with mucosal viral control. Am J Transplant. 2013 Jan;13(1):146–156.
Journal cover image

Published In

Am J Transplant

DOI

EISSN

1600-6143

Publication Date

January 2013

Volume

13

Issue

1

Start / End Page

146 / 156

Location

United States

Related Subject Headings

  • Surgery
  • Prospective Studies
  • Middle Aged
  • Male
  • Lung Transplantation
  • Interferon-gamma
  • Immunologic Memory
  • Immunity, Mucosal
  • Humans
  • Flow Cytometry