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GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae.

Publication ,  Journal Article
Kiktev, DA; Sheng, Z; Lobachev, KS; Petes, TD
Published in: Proc Natl Acad Sci U S A
July 24, 2018

The chromosomes of many eukaryotes have regions of high GC content interspersed with regions of low GC content. In the yeast Saccharomyces cerevisiae, high-GC regions are often associated with high levels of meiotic recombination. In this study, we constructed URA3 genes that differ substantially in their base composition [URA3-AT (31% GC), URA3-WT (43% GC), and URA3-GC (63% GC)] but encode proteins with the same amino acid sequence. The strain with URA3-GC had an approximately sevenfold elevated rate of ura3 mutations compared with the strains with URA3-WT or URA3-AT About half of these mutations were single-base substitutions and were dependent on the error-prone DNA polymerase ζ. About 30% were deletions or duplications between short (5-10 base) direct repeats resulting from DNA polymerase slippage. The URA3-GC gene also had elevated rates of meiotic and mitotic recombination relative to the URA3-AT or URA3-WT genes. Thus, base composition has a substantial effect on the basic parameters of genome stability and evolution.

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Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 24, 2018

Volume

115

Issue

30

Start / End Page

E7109 / E7118

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Base Sequence
  • Base Composition
 

Citation

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Kiktev, D. A., Sheng, Z., Lobachev, K. S., & Petes, T. D. (2018). GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A, 115(30), E7109–E7118. https://doi.org/10.1073/pnas.1807334115
Kiktev, Denis A., Ziwei Sheng, Kirill S. Lobachev, and Thomas D. Petes. “GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae.Proc Natl Acad Sci U S A 115, no. 30 (July 24, 2018): E7109–18. https://doi.org/10.1073/pnas.1807334115.
Kiktev DA, Sheng Z, Lobachev KS, Petes TD. GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7109–18.
Kiktev, Denis A., et al. “GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae.Proc Natl Acad Sci U S A, vol. 115, no. 30, July 2018, pp. E7109–18. Pubmed, doi:10.1073/pnas.1807334115.
Kiktev DA, Sheng Z, Lobachev KS, Petes TD. GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):E7109–E7118.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 24, 2018

Volume

115

Issue

30

Start / End Page

E7109 / E7118

Location

United States

Related Subject Headings

  • Sequence Deletion
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Base Sequence
  • Base Composition