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Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila.

Publication ,  Journal Article
Barish, S; Volkan, PC
Published in: Journal of visualized experiments : JoVE
June 2018

The olfactory system of Drosophila is a widely used system in developmental neurobiology, systems neuroscience, as well as neurophysiology, behavior, and behavioral evolution. Drosophila olfactory tissues house the olfactory receptor neurons (ORNs) that detect volatile chemical cues in addition to hydro- and thermo-sensory neurons. In this protocol, we describe the dissection of developing peripheral olfactory tissue of the adult Drosophila species. We first describe how to stage and age Drosophila larvae, followed by the dissection of the antennal disc from early pupal stages, followed by the dissection of the antennae from mid-pupal stages and adults. We also show methods where preparations can be utilized in molecular techniques, such as the RNA extraction for qRT-PCR, RNAseq, or immunohistochemistry. These methods can also be applied to other Drosophila species after species-specific pupal development times are determined, and respective stages are calculated for appropriate aging.

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Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

June 2018

Issue

136

Related Subject Headings

  • Olfactory Receptor Neurons
  • Olfactory Pathways
  • Immunohistochemistry
  • Drosophila Proteins
  • Drosophila
  • Animals
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences
  • 1701 Psychology
  • 0601 Biochemistry and Cell Biology
 

Citation

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ICMJE
MLA
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Barish, S., & Volkan, P. C. (2018). Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila. Journal of Visualized Experiments : JoVE, (136). https://doi.org/10.3791/57716
Barish, Scott, and Pelin C. Volkan. “Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila.Journal of Visualized Experiments : JoVE, no. 136 (June 2018). https://doi.org/10.3791/57716.
Barish S, Volkan PC. Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila. Journal of visualized experiments : JoVE. 2018 Jun;(136).
Barish, Scott, and Pelin C. Volkan. “Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila.Journal of Visualized Experiments : JoVE, no. 136, June 2018. Epmc, doi:10.3791/57716.
Barish S, Volkan PC. Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila. Journal of visualized experiments : JoVE. 2018 Jun;(136).

Published In

Journal of visualized experiments : JoVE

DOI

EISSN

1940-087X

ISSN

1940-087X

Publication Date

June 2018

Issue

136

Related Subject Headings

  • Olfactory Receptor Neurons
  • Olfactory Pathways
  • Immunohistochemistry
  • Drosophila Proteins
  • Drosophila
  • Animals
  • 3101 Biochemistry and cell biology
  • 1702 Cognitive Sciences
  • 1701 Psychology
  • 0601 Biochemistry and Cell Biology