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Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans.

Publication ,  Journal Article
Son, Y-E; Jung, W-H; Oh, S-H; Kwak, J-H; Cardenas, ME; Park, H-S
Published in: Mycobiology
2018

Mon1 is a guanine nucleotide exchange factor subunit that activates the Ypt7 Rab GTPase and is essential for vacuole trafficking and autophagy in eukaryotic organisms. Here, we identified and characterized the function of Mon1, an ortholog of Saccharomyces cerevisiae Mon1, in a human fungal pathogen, Cryptococcus neoformans. Mutation in mon1 resulted in hypersensitivity to thermal stress. The mon1 deletion mutant exhibited increased sensitivity to cell wall and endoplasmic reticulum stress. However, the mon1 deletion mutant showed more resistance to the antifungal agent fluconazole. In vivo studies demonstrated that compared to the wild-type strain, the mon1 deletion mutant attenuated virulence in the Galleria mellonella insect model. Moreover, the mon1 deletion mutant was avirulent in the murine inhalation model. These results demonstrate that Mon1 plays a crucial role in stress survival and pathogenicity in C. neoformans.

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Published In

Mycobiology

DOI

ISSN

1229-8093

Publication Date

2018

Volume

46

Issue

2

Start / End Page

114 / 121

Location

Korea (South)
 

Citation

APA
Chicago
ICMJE
MLA
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Son, Y.-E., Jung, W.-H., Oh, S.-H., Kwak, J.-H., Cardenas, M. E., & Park, H.-S. (2018). Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans. Mycobiology, 46(2), 114–121. https://doi.org/10.1080/12298093.2018.1468053
Son, Ye-Eun, Won-Hee Jung, Sang-Hun Oh, Jin-Hwan Kwak, Maria E. Cardenas, and Hee-Soo Park. “Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans.Mycobiology 46, no. 2 (2018): 114–21. https://doi.org/10.1080/12298093.2018.1468053.
Son Y-E, Jung W-H, Oh S-H, Kwak J-H, Cardenas ME, Park H-S. Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans. Mycobiology. 2018;46(2):114–21.
Son, Ye-Eun, et al. “Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans.Mycobiology, vol. 46, no. 2, 2018, pp. 114–21. Pubmed, doi:10.1080/12298093.2018.1468053.
Son Y-E, Jung W-H, Oh S-H, Kwak J-H, Cardenas ME, Park H-S. Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans. Mycobiology. 2018;46(2):114–121.

Published In

Mycobiology

DOI

ISSN

1229-8093

Publication Date

2018

Volume

46

Issue

2

Start / End Page

114 / 121

Location

Korea (South)